|Sample Size:||30ug for $99, contact us for details|
|Application:||ELISA, IHC, WB|
Data & Images
|Product Name||Anti-Growth Hormone Antibody|
|Description||Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA in Rat.|
|Cite This Product||Anti-Growth Hormone Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1007)|
|Replacement Item||This antibody may replace the following items: sc-133742|sc-32245|sc-5638|sc-5640|sc-5639|sc-5637|sc-8185|sc-28788|sc-374324|sc-374300|sc-374334|sc-8184|sc-133742|sc-271924|sc-28465-R|sc-28788|sc-32245|sc-374300|sc-374324|sc-374334|sc-5638|sc-8184|sc-8185 from Santa Cruz Biotechnology.|
|Application||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E. coli-derived rat GH recombinant protein(Position: F27-F216).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||24656 MW|
|Protein Function||Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.|
|Alternative Names||Somatotropin;Growth hormone;Gh1;Gh;|
|Research Areas|||signal transduction|growth factors/hormones| developmental biology|post embryonic development|growth hormones||
Background for Somatotropin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Growth Hormone Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Rat, -
Western blot, 0.1-0.5μg/ml, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Growth Hormone Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Rat GH Protein 10ng
Lane 2: Recombinant Rat GH Protein 5ng
Lane 3: Recombinant Rat GH Protein 2
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,