Anti-Rhodopsin Monoclonal Antibody

Rhodopsin antibody

Boster Bio Anti-Rhodopsin Monoclonal Antibody catalog # M00083-1. Tested in ELISA, IP, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00083-1
Size: 100ug
Reactive Species: Mammals, Amphibians, Fish, Shark, Avian
Host: Mouse
Application: ELISA, IP, IF, IHC, ICC, WB

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Product Name

Anti-Rhodopsin Monoclonal Antibody

View all Rhodopsin Antibodies

SKU/Catalog Number

M00083-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-Rhodopsin Monoclonal Antibody catalog # M00083-1. Tested in ELISA, IP, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Rhodopsin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00083-1)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

4D2

Isotype

IgG1

Immunogen

Bovine Rhodopsin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Reactive Species

M00083-1 is reactive to RHO in Mammals, Amphibians, Fish, Shark, Avian

Applications

M00083-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

38.893kDa

Background of Rhodopsin

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For RHO (Source: Uniprot.org, NCBI)

Gene Name

RHO

Full Name

Rhodopsin

Weight

38.893kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

CSNBAD1; MGC138309; OPN2MGC138311; opsin 2, rod pigment; opsin-2; retinitis pigmentosa 4, autosomal dominant; rhodopsin; RP4 RHO CSNBAD1, OPN2, RP4 rhodopsin rhodopsin|opsin 2, rod pigment|opsin-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RHO, check out the RHO Infographic

RHO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RHO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00083-1

100 μg

$416

Free Secondary Antibody

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M00083-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$416.00

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