|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-RIAM Antibody|
|Description||Rabbit IgG polyclonal antibody for Amyloid beta A4 precursor protein-binding family B member 1-interacting protein(APBB1IP) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Rat.|
|Cite This Product||Anti-RIAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1960)|
|Replacement Item||This antibody may replace the following items: sc-104813 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP(647-666aa EQDFMSDLMKALQKKRGNVS), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Amyloid beta A4 precursor protein-binding family B member 1-interacting protein|
|Molecular Weight||73183 MW|
|Protein Function||Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion. .|
|Tissue Specificity||Widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. .|
|Sequence Similarities||Belongs to the MRL family.|
|Subcellular Localization||Cell membrane ; Peripheral membrane protein . Cell projection, lamellipodium . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F- actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases (By similarity). .|
|Alternative Names||Amyloid beta A4 precursor protein-binding family B member 1-interacting protein;APBB1-interacting protein 1;Proline-rich EVH1 ligand 1;PREL-1;Proline-rich protein 73;Rap1-GTP-interacting adapter molecule;RIAM;Retinoic acid-responsive proline-rich protein 1;RARP-1;APBB1IP;PREL1, RARP1, RIAM;|
Background for Amyloid beta A4 precursor protein-binding family B member 1-interacting protein
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-RIAM Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Rat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-RIAM Antibody Images
Click the images to enlarge.
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat RAJI Tissue Lysate
Lane 4: HL-60 Cell Lysate
IHC(P): Human Tonsil Tissue
ICC: JURKAT Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,