Data & Images
|Product Name||Anti-RIP3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 3(RIPK3) detection. Tested with WB in Human.|
|Cite This Product||Anti-RIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2242)|
|Replacement Item||This antibody may replace the following items: sc-135170|sc-135171|sc-374639|sc-47368|sc-56228 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3(60-76aa EVKAMASLDNEFVLRLE).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Receptor-interacting serine/threonine-protein kinase 3|
|Molecular Weight||56887 MW|
|Protein Function||Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. .|
|Tissue Specificity||Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.|
|Subcellular Localization||Cytoplasm, cytosol . Cell membrane . Mitochondrion .|
|Alternative Names||Receptor-interacting serine/threonine-protein kinase 3;184.108.40.206;RIP-like protein kinase 3;Receptor-interacting protein 3;RIP-3;RIPK3;RIP3;|
|Research Areas|||cell biology|apoptosis|receptors|associated proteins| cell biology|intracellular|kinases| signal transduction|protein phosphorylation|ser / thr kinases|other kinases| immunology|innate immunity|tlr signaling| cancer|cell death|necroptosis||
Background for Receptor-interacting serine/threonine-protein kinase 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-RIP3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-RIP3 Antibody Images
Click the images to enlarge.
Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,