Overview
| Product Name |
Anti-ROBO-1 Antibody
|
| SKU/Catalog Number |
A01530-1 |
| Pack Size |
100ug |
| Storage & Handling |
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Description |
Rabbit Polyclonal antibody for ROBO1 detection. Tested positive for ELISA,Immunohistochemistry,IF Microscopy,Western Blot, in human, mouse, rat and dog |
| Cite This Product |
Anti-ROBO-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1. |
Gene/Protein Basic Information For ROBO1 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q2M1J3 |
|---|
| Gene Name | ROBO1 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ROBO1 (Source: Uniprot.org, NCBI)
Our Boster Quality Guarantee for Anti-ROBO-1 Antibody covers its use in the following applications.
IF Microscopy: User Optimized
Western Blot: 1:500 - 1:3,000
Immunohistochemistry: 2 ug/ml to 10 ug/ml
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 4. IHC analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
ROBO1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROBO1 Antibody (A01530-1) overnight at 4
Affinity Purified anti-ROBO5
ELISA results of purified Rabbit anti-Robo-immunizing peptide. Each well was coated in duplicate with 0.1
Staining of ROBO1/200. DAPI was used for nuclear counterstaining.Image from Lindenmeyer MT et al.Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7):e11545, Fig 5.
Figure 7. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Figure 3. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Figure 2. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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