SKU A01530-1
Size 100ug
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications IF, IHC, WB

Overview

Product Name Anti-ROBO-1 Antibody
SKU/Catalog Number A01530-1
Storage & Handling Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Size 100ug
Description Rabbit Polyclonal antibody for ROBO1 detection. Tested positive for ELISA,Immunohistochemistry,IF Microscopy,Western Blot, in human, mouse, rat and dog
Cite This Product Anti-ROBO-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-1)
Host Rabbit
Contents/Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
IF Microscopy: User Optimized
Western Blot: 1:500 - 1:3,000
Immunohistochemistry: 2 ug/ml to 10 ug/ml

Images And Assay Conditions

/A/0/A01530 1 ROBO1 IHC test 4.jpg

Figure 4. IHC analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
ROBO1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROBO1 Antibody (A01530-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A01530 1 ROBO1 IHC test 1.jpg

Affinity Purified anti-ROBO5

/A/0/A01530 1 ROBO1 elisa test 6.jpg

ELISA results of purified Rabbit anti-Robo-immunizing peptide. Each well was coated in duplicate with 0.1

/A/0/A01530 1 ROBO1 ICC test 5.jpg

Staining of ROBO1/200. DAPI was used for nuclear counterstaining.Image from Lindenmeyer MT et al.Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7):e11545, Fig 5.

/A/0/A01530 1 ROBO1 WB test 7.jpg

Figure 7. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ROBO1.

/A/0/A01530 1 ROBO1 WB test 3.jpg

Figure 3. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ROBO1.

/A/0/A01530 1 ROBO1 WB test 2.jpg

Figure 2. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ROBO1.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q2M1J3
Gene Name ROBO1

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Angiogenesis, Axonal Guidance Proteins, Cancer, Growth And Development, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience

*You can search these to find other products in these research areas.

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Order Product (A01530-1)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $349
100ug+Free HRP Secondary BA1054 $349
100ug+Free Biotin Secondary BA1003 $349

USD $349

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