Overview
| Product Name |
Anti-ROBO-1 Antibody
|
| Catalog# |
A01530-1 |
| Pack Size |
100ug |
| Storage & Handling |
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Description |
Rabbit Polyclonal antibody for ROBO1 detection. Tested positive for ELISA,Immunohistochemistry,IF Microscopy,Western Blot, in human, mouse, rat and dog |
| Cite This Product |
Anti-ROBO-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01530-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1. |
Gene/Protein Basic Information For ROBO1 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q2M1J3 |
|---|
| Gene Name | ROBO1 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ROBO1, check out the ROBO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ROBO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ROBO1 infographic
Our Boster Quality Guarantee for Anti-ROBO-1 Antibody covers its use in the following applications.
IF Microscopy: User Optimized
Western Blot: 1:500 - 1:3,000
Immunohistochemistry: 2 ug/ml to 10 ug/ml
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 4. IHC analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
ROBO1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ROBO1 Antibody (A01530-1) overnight at 4
Affinity Purified anti-ROBO5
ELISA results of purified Rabbit anti-Robo-immunizing peptide. Each well was coated in duplicate with 0.1
Staining of ROBO1/200. DAPI was used for nuclear counterstaining.Image from Lindenmeyer MT et al.Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7):e11545, Fig 5.
Figure 7. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Figure 3. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Figure 2. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-1) at 0.5 ug/mL overnight at 4
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Other Recommended Resources
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Total number of citations: 0
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Questions and answers from customers related to A01530-1 Anti-ROBO-1 Antibody
6 Related Questions
Question
Will A01530-1 anti-ROBO-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2020-02-24
Answer
It shows on the product datasheet, A01530-1 anti-ROBO-1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-24
Question
Is this A01530-1 anti-ROBO-1 antibody reactive to the isotypes of ROBO1?
Verified Customer
Asked: 2020-01-28
Answer
The immunogen of A01530-1 anti-ROBO-1 antibody is This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1632-1644 of Human ROBO-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-ROBO-1 antibody A01530-1. Let me know if you need anything else.
Verified Customer
Asked: 2019-12-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
Is a blocking peptide available for product anti-ROBO-1 antibody (A01530-1)?
Verified Customer
Asked: 2018-06-21
Answer
We do provide the blocking peptide for product anti-ROBO-1 antibody (A01530-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-21
Question
See below the WB image, lot number and protocol we used for forebrain using anti-ROBO-1 antibody A01530-1. Please let me know if you require anything else.
K. Bhatt
Asked: 2014-05-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-05-27
Question
Our lab want to know about to test anti-ROBO-1 antibody A01530-1 on human forebrain for research purposes, then I may be interested in using anti-ROBO-1 antibody A01530-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Carter
Asked: 2014-03-03
Answer
The products we sell, including anti-ROBO-1 antibody A01530-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-03
