Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-ROCK2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-ROCK2 Antibody
See all ROCK2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for ROCK2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ROCK2 information: Molecular Weight: 160900 MW; Subcellular Localization: Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.|
|Cite This Product||Anti-ROCK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1242)|
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|Anti-ROCK2 Antibody may replace the following items: sc 100425|sc 1851|sc 1852|sc 365275|sc 398519|sc 5561.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human ROCK2(35-51aa RDPRSPINVESLLDGLN), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ROCK2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||9475|
|Species Of This Entry||Human|
|Protein Name||Rho-associated protein kinase 2|
|Superfamily||protein kinase superfamily|
|Alternative Names||ROCK2|coiled-coil-containing protein kinase 2; EC 2.7.11; EC 188.8.131.52; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK2; ROCK-II; ROKalpha|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Amidation; Methylation; Geranylgeranylation; Ubiquitination|
|Subcellular Localization||Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.|
|Gene Location||2p25.1, on Chromasome 2, gene sequence: NC_000002.12 (11179759..11345437, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ROCK2 (Source: Uniprot.org, NCBI)
|Protein Function||Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.|
|Interacting Proteins||JAK2; STAT3|
|Research Areas||Breast Cancer; Cancer; Protein Kinase; Wnt Signaling Pathway|
|Related Diseases||Tissue Adhesions; Malignant Neoplasms; Hypertensive Disease; Neoplasms; Cardiovascular Diseases; Cell Invasion; Inflammation; Carcinoma; Neoplasm Metastasis; Pathologic Vasoconstriction; Neoplasm Invasiveness; Pulmonary Hypertension; Atherosclerosis; Fibrosis; Hypoxia; Hypertrophy; Cell Transformation, Neoplastic; Malignant Squamous Cell Neoplasm; Malignant Neoplasm Of Breast; Mammary Neoplasms|
|Related Pathways||Cell Migration; Pathogenesis; Cell Motility; Cell Adhesion; Vasoconstriction; Cell Proliferation; Cell Cycle; Cytokinesis; Sensitization; Angiogenesis; Muscle Contraction; Smooth Muscle Contraction; Cell Death; Localization; Secretion; Cell Differentiation; Translation; Cell Growth; Regeneration; Transport|
|Background||Rho-associated kinase(ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al.(2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.|
|Scientific References||PMID: 9933571 by Takahashi N., et al. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.|
PMID: 10579722 by Kawano Y., et al. Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo.
Our Boster Quality Guarantee for Anti-ROCK2 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: MM453 Cell Lysate
IHC(P): Human Rectal Cancer Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # PA1242) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161KD. The expected band size for ROCK2 is at 161KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Tang Yh, Yang Js, Xiang Hy, Xu Jj. Clin Invest Med. 2014 Feb 1;37(1):E26-37. Pi3K-Akt/Enos In Remote Postconditioning Induced By Brief Pulmonary Ischemia.||PubMed ID 24502809|
|Wang Hm, Wang Y, Liu M, Bai Y, Zhang Xh, Sun Yx, Wang Hl. Can J Physiol Pharmacol. 2012 Nov;90(11):1506-15. Doi: 10.1139/Y2012-108. Epub 2012 Nov 8. Fluoxetine Inhibits Monocrotaline-Induced Pulmonary Arterial Remodeling Involved In Inhibition Of ...||PubMed ID 23181278|
|The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia||PubMed ID 26604905|
|8,9-Epoxyeicosatrienoic acid analog protects pulmonary artery smooth muscle cells from apoptosis via ROCK pathway||PubMed ID 20493836|
|ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension||PubMed ID 27284349|
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Note: sample size takes longer to process, average 5-7 days.
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