Anti-ROCK2 Antibody

Boster Bio Anti-ROCK2 Antibody catalog # PA1242. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: PA1242
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-ROCK2 Antibody

See all ROCK2 products

SKU/Catalog Number

PA1242

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ROCK2 Antibody catalog # PA1242. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ROCK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1242)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ROCK2 (35-51aa RDPRSPINVESLLDGLN), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1242 is reactive to ROCK2 in Human, Mouse, Rat

Applications

PA1242 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ROCK2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O75116

Gene ID

9475

Gene Name

ROCK2

Full Name

Rho-associated protein kinase 2

Weight

160.9kDa

Superfamily

protein kinase superfamily

Alternative Names

coiled-coil-containing protein kinase 2; EC 2.7.11; EC 2.7.11.1; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK2; ROCK-II; ROKalpha ROCK2 ROCK-II Rho associated coiled-coil containing protein kinase 2 rho-associated protein kinase 2|p164 ROCK-2|rho-associated, coiled-coil-containing protein kinase II

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ROCK2, check out the ROCK2 Infographic

ROCK2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ROCK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1242 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Han F,Xu H,Shen JX,Pan C,Yu ZH,Chen JJ,Zhu XL,Cai YF,Lu YP.RhoA/Rock2/Limk1/cofilin1 pathway is involved in attenuation of neuronal dendritic spine loss by paeonol in the frontal cortex of D-galactose and aluminum‑induced Alzheimer‘s disease‑like ra
Species: Rat
PA1242 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:250

ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension

8,9-Epoxyeicosatrienoic acid analog protects pulmonary artery smooth muscle cells from apoptosis via ROCK pathway

The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia

Wang Hm, Wang Y, Liu M, Bai Y, Zhang Xh, Sun Yx, Wang Hl. Can J Physiol Pharmacol. 2012 Nov;90(11):1506-15. Doi: 10.1139/Y2012-108. Epub 2012 Nov 8. Fluoxetine Inhibits Monocrotaline-Induced Pulmonary Arterial Remodeling Involved In Inhibition Of ...

Tang Yh, Yang Js, Xiang Hy, Xu Jj. Clin Invest Med. 2014 Feb 1;37(1):E26-37. Pi3K-Akt/Enos In Remote Postconditioning Induced By Brief Pulmonary Ischemia.

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7 Customer Q&As for Anti-ROCK2 Antibody

Question

Will anti-ROCK2 antibody PA1242 work for WB with liver?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

According to the expression profile of liver, ROCK2 is highly expressed in liver. So, it is likely that anti-ROCK2 antibody PA1242 will work for WB with liver.

Boster Scientific Support

Answered: 2020-05-07

Question

I have attached the WB image, lot number and protocol we used for liver using anti-ROCK2 antibody PA1242. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-20

Question

Do you have a BSA free version of anti-ROCK2 antibody PA1242 available?

O. Anderson

Verified customer

Asked: 2019-12-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ROCK2 antibody PA1242 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-31

Question

I was wanting to use your anti-ROCK2 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

It shows on the product datasheet, PA1242 anti-ROCK2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ROCK2 antibody PA1242. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-12-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-14

Question

Does anti-ROCK2 antibody PA1242 work on pig WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2018-01-02

Answer

Our lab technicians have not tested anti-ROCK2 antibody PA1242 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ROCK2 antibody PA1242 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-02

Question

Is a blocking peptide available for product anti-ROCK2 antibody (PA1242)?

V. Parker

Verified customer

Asked: 2013-02-13

Answer

We do provide the blocking peptide for product anti-ROCK2 antibody (PA1242). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-02-13

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1242
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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