SKU A08104-1
Size 100 ul
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications WB

Overview

Product Name Anti-RRAD Antibody
SKU/Catalog Number A08104-1
Storage & Handling -20°C
Size 100 ul
Description Anti-RRAD Antibody tested in WB|WB, reactive to human|mouse|rat
Cite This Product Anti-RRAD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08104-1)
Host Rabbit
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RRAD
Reactivity Human, Mouse, Rat

Assay Details

Images And Assay Conditions

/a/0/a08104 1 wb anti rrad antibody figure 1.jpg

Figure 1. Western blot analysis of RRAD using anti-RRAD antibody (A08104-1).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human Adult Placenta

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRAD antigen affinity purified polyclonal antibody (Catalog # A08104-1) at 1.0ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRAD. The expected band size for RRAD is at 33.2KD.

/a/0/a08104 1 wb anti rrad antibody figure 2.jpg

Figure 2. Western blot analysis of RRAD using anti-RRAD antibody (A08104-1).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: HepG2 cell lysate

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRAD antigen affinity purified polyclonal antibody (Catalog # A08104-1) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRAD. The expected band size for RRAD is at 33.2KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P55042
Gene Name RRAD
Protein Name GTP-binding protein RAD
Tissue Specificity Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
Alternative Names GTP-binding protein RAD;RAD1;Ras associated with diabetes;RRAD;RAD;
Subcellular Localization Cell membrane .
Molecular Weight 33245 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha- 1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
Research Areas Cardiovascular, G Protein Signaling, Hypertrophy, Signal Transduction, Signaling Pathway, Small G Proteins

*You can search these to find other products in these research areas.

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