Data & Images
|Product Name||Anti-RUNX1/AML1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Runt-related transcription factor 1(RUNX1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-RUNX1/AML1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9157)|
|Replacement Item||This antibody may replace the following items: sc-8563|sc-28679|sc-101146|sc-8564|sc-365644|sc-28679-X|sc-8564-X|sc-365644-X|sc-8563-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human RUNX1(200-233aa ELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFN), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Runt-related transcription factor 1|
|Molecular Weight||48737 MW|
|Protein Function||CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). .|
|Tissue Specificity||Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.|
|Sequence Similarities||Contains 1 Runt domain.|
|Alternative Names||Runt-related transcription factor 1;Acute myeloid leukemia 1 protein;Core-binding factor subunit alpha-2;CBF-alpha-2;Oncogene AML-1;Polyomavirus enhancer-binding protein 2 alpha B subunit;PEA2-alpha B;PEBP2-alpha B;SL3-3 enhancer factor 1 alpha B subunit;SL3/AKV core-binding factor alpha B subunit;RUNX1;AML1, CBFA2;|
|Research Areas|||neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|other factors| stem cells|hematopoietic progenitors|intracellular molecules|transcription factors|chromatin binding proteins|dna / rna binding|mesenchymal stem cells|osteogenesis| cancer|oncoproteins/suppressors|oncoproteins|hemangioblast| developmental biology|organogenesis|hematopoietic system development|skeletal development|bone||
Background for Runt-related transcription factor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-RUNX1/AML1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-RUNX1/AML1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti RUNX1 (PB9157) at 0.5ug/ml
WB: Recombinant Human RUNX1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
IHC(P): Rat Thymus Tissue
IHC(P): Human Mammary Cancer Tissue
IHC(P): Mouse Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,