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SKU:MA1088
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:SH-B1
Application:IHC-P
Price: $200.00
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Overview

Product Name Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)
SKU/Catalog Number MA1088
Description Mouse IgG monoclonal antibody for S-100 (beta-subunit), S100 calcium binding protein B (S100B) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) SH-B1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)
Replacement Item This antibody may replace the following items: sc-136061|sc-28533|sc-393919|sc-398707|sc-52204|sc-52205|sc-52206|sc-58839|sc-71990|sc-71991|sc-7851|sc-7852|sc-81709 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rabbit, Rat
Predicted Species Horse, Monkey, Rabbit

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Bovine brain S-100b.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number SH-B1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name S100b
Protein Name Protein S100-B
Molecular Weight 10744 MW
Protein Function Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .
Tissue Specificity Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .
Sequence Similarities Belongs to the S-100 family.
Subcellular Localization Cytoplasm . Nucleus .
Uniprot ID P04631
Alternative Names Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;
Research Areas |cell biology|cell cycle|cell differentiation| epigenetics and nuclear signaling| stem cells|neural stem cells|glial restricted lineage|astrocyte|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Protein S100-B

S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) Images

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Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)
Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P)
IHC(P): Rat Brain Tissue Lysate
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Publications

Yi M, Yang J, Chen X, Li J, Li X, Wang L, Tan Y, Xiong W, Zhou M, Mccarthy Jb, Li G, Xiang B, Xie H. J Cell Physiol. 2011 Sep;226(9):2360-9. Doi: 10.1002/Jcp.22568. Rassf1A Suppresses Melanoma Development By Modulating Apoptosis And Cell-Cycle Pro...
Bao Hj, Liu Y, Qin Jh, Xu Cs, Hei Nn, Jaber Jr, Chen Qs. Res Vet Sci. 2011 Dec;91(3):E16-24. Doi: 10.1016/J.Rvsc.2011.02.015. Epub 2011 Apr 12. An Immunohistochemical Study Of S-100 Protein In The Intestinal Tract Of Chinese Soft-Shelled Turtle, P...
Chen Hx, Tang Sp, Gao Ft, Xu Jl, Jiang Xp, Cao J, Fu Gb, Sun K, Liu Sz, Shi W. Medicine (Baltimore). 2014 Nov;93(23):E138. Doi: 10.1097/Md.0000000000000138. Fibrosis, Adipogenesis, And Muscle Atrophy In Congenital Muscular Torticollis.
Jin X, Chen Z, Liu X, Liang B, Zhang H, Zhang Z. Brain Res. 2014 Apr 16;1558:84-9. Doi: 10.1016/J.Brainres.2014.02.032. Epub 2014 Feb 22. The Expression Of Endothelial Barrier Antigen (Eba) And S100B In The Rat Parietal Cortex Following Brain Irra...
Yang Lm, Liu Xl, Zhu Qt, Zhang Y, Xi Tf, Hu J, He Cf, Jiang L. J Biomed Mater Res B Appl Biomater. 2011 Jan;96(1):25-33. Doi: 10.1002/Jbm.B.31719. Human Peripheral Nerve-Derived Scaffold For Tissue-Engineered Nerve Grafts: Histology And Biocompati...
Sun D, Li X, Ma M, Liu J, Xu Y, Ye L, Hou H, Wang C, Li X, Jiang Y. Jpn J Clin Oncol. 2015 May;45(5):464-73. Doi: 10.1093/Jjco/Hyv009. Epub 2015 Feb 2. The Predictive Value And Potential Mechanisms Of Mirna-328 And Mirna-378 For Brain Metastases I...
Guo Js, Zeng Ys, Li Hb, Huang Wl, Liu Ry, Li Xb, Ding Y, Wu Lz, Cai Dz. Spinal Cord. 2007 Jan;45(1):15-24. Epub 2006 Jun 13. Cotransplant Of Neural Stem Cells And Nt-3 Gene Modified Schwann Cells Promote The Recovery Of Transected Spinal Cord Injury.
Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.
Zhao F, He W, Zhang Y, Tian D, Zhao H, Yu K, Bai J. Neural Regen Res. 2013 Jul 25;8(21):1974-84. Doi: 10.3969/J.Issn.1673-5374.2013.21.006. Electric Stimulation And Decimeter Wave Therapy Improve The Recovery Of Injured Sciatic Nerves.
Zhang Gq, Yang S, Li Xs, Zhou Ds. Acta Histochem. 2014 Jun;116(5):788-94. Doi: 10.1016/J.Acthis.2014.01.011. Epub 2014 Mar 12. Expression And Possible Role Of Igf-Ir In The Mouse Gastric Myenteric Plexus And Smooth Muscles.
Yang P, Gao Z, Zhang H, Fang Z, Wu C, Xu H, Huang Qj. Neuropsychiatr Dis Treat. 2015 Mar 6;11:597-607. Doi: 10.2147/Ndt.S78131. Ecollection 2015. Changes In Proinflammatory Cytokines And White Matter In Chronically Stressed Rats.
He L, Zhang Y, Zeng C, Ngiam M, Liao S, Quan D, Zeng Y, Lu J, Ramakrishna S. Tissue Eng Part C Methods. 2009 Jun;15(2):243-55. Doi: 10.1089/Ten.Tec.2008.0255. Manufacture Of Plga Multiple-Channel Conduits With Precise Hierarchical Pore Architectur...
Su Z, Chen J, Qiu Y, Yuan Y, Zhu F, Zhu Y, Liu X, Pu Y, He C. Glia. 2013 Apr;61(4):490-503. Doi: 10.1002/Glia.22450. Epub 2013 Jan 22. Olfactory Ensheathing Cells: The Primary Innate Immunocytes In The Olfactory Pathway To Engulf Apoptotic Olfacto...
Su Z, Yuan Y, Chen J, Cao L, Zhu Y, Gao L, Qiu Y, He C. Plos One. 2009 Dec 3;4(12):E8141. Doi: 10.1371/Journal.Pone.0008141. Reactive Astrocytes In Glial Scar Attract Olfactory Ensheathing Cells Migration By Secreted Tnf-Alpha In Spinal Cord Lesio...
Sun Lq, Xue B, Li Xj, Wang X, Qu L, Zhang Tt, Zhao J, Wang Ba, Zou Xm, Mu Ym, Lu Jm. Life Sci. 2012 Jan 16;90(3-4):99-108. Doi: 10.1016/J.Lfs.2011.10.001. Epub 2011 Oct 20. Inhibitory Effects Of Salvianolic Acid B On Apoptosis Of Schwann Cells And...
Xiong Hl, Guo Zy, Li Sx, Li N, Liu Sh, Ji Yh. Phys Med Biol. 2013 Mar 7;58(5):1549-61. Doi: 10.1088/0031-9155/58/5/1549. Epub 2013 Feb 15. In Vivo Detection Of Hirschsprung'S Disease By Optical Coherence Tomography In Rats.
Sun T, Ye C, Zhang Z, Wu J, Huang H. Cell Transplant. 2013;22 Suppl 1:S27-38. Doi: 10.3727/096368913X672118. Epub 2013 Aug 29. Cotransplantation Of Olfactory Ensheathing Cells And Schwann Cells Combined With Treadmill Training Promotes Functional ...
Qu L, Liang X, Gu B, Liu W. Neural Regen Res. 2014 Jun 15;9(12):1195-203. Doi: 10.4103/1673-5374.135328. Quercetin Alleviates High Glucose-Induced Schwann Cell Damage By Autophagy.
Wu Y, Xue B, Li X, Liu H. Neural Regen Res. 2012 Nov 25;7(33):2583-91. Doi: 10.3969/J.Issn.1673-5374.2012.33.003. Puerarin Prevents High Glucose-Induced Apoptosis Of Schwann Cells By Inhibiting Oxidative Stress.
Xue B, Sun L, Li X, Wang X, Zhang Y, Mu Y, Liang L. Neural Regen Res. 2012 Oct 25;7(30):2340-6. Doi: 10.3969/J.Issn.1673-5374.2012.30.003. Ginsenoside Rb1 Relieves Glucose Fluctuation-Induced Oxidative Stress And Apoptosis In Schwann Cells.
Tang J, Li Q, Cheng B, Huang C, Chen K. Biomed Rep. 2015 Jul;3(4):499-502. Epub 2015 May 27. Effects Of Diethylstilbestrol On The Proliferation And Tyrosinase Activity Of Cultured Human Melanocytes.
Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...
Zhang C, Peng Y, Wang F, Tan X, Liu N, Fan S, Wang D, Zhang L, Liu D, Wang T, Wang S, Zhou Y, Su Y, Cheng T, Zhuang Z, Shi C. Biomaterials. 2010 Dec;31(36):9535-43. Doi: 10.1016/J.Biomaterials.2010.08.059. Epub 2010 Sep 26. A Synthetic Cantharidin...
Liu S, Zhang Y, Zhao Y, Cui H, Cao C, Guo J. Cell Biochem Biophys. 2015 Jan;71(1):401-6. Doi: 10.1007/S12013-014-0212-1. Effects Of Hypothermia On S100B And Glial Fibrillary Acidic Protein In Asphyxia Rats After Cardiopulmonary Resuscitation.
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...
Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: Can you clarify the statement on this page "No cross reactivity with other proteins"?
A: It means the antibody only recognizes S-100 beta and does not cross-react with any other cytokines.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to s100 antibody, s100b antibody, nef antibody