Product Info Summary
SKU: | M02503 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF |
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Product info
Product Name
Anti-S100A (Melanoma Marker) S100A1 Monoclonal Antibody
SKU/Catalog Number
M02503
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-S100A (Melanoma Marker) S100A1 Monoclonal Antibody (Catalog # M02503). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-S100A (Melanoma Marker) S100A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02503)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: S1/61
Isotype
IgG1, kappa
Immunogen
Purified human full-length native S-100 protein conjugated to methylated BSA
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02503 is reactive to S100A1 in Human
Applications
M02503 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.546kDa
Background of S100a1
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-S100A1 Mouse Monoclonal Antibody (S1/61)
Click image to see more details
Flow Cytometric analysis of T98G cells using Anti-S100A1 Mouse Monoclonal Antibody (S1/61) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For S100A1 (Source: Uniprot.org, NCBI)
Gene Name
S100A1
Full Name
Protein S100-A1
Weight
10.546kDa
Superfamily
S-100 family
Alternative Names
protein S100-A1; S100 alpha; S100 calcium binding protein A1; S100 calcium-binding protein A1S100; S-100 protein alpha chain; S-100 protein subunit alpha; S100 protein, alpha polypeptide; S100A; S100A1; S100-alpha S100A1 S100, S100-alpha, S100A S100 calcium binding protein A1 protein S100-A1|S-100 protein alpha chain|S-100 protein subunit alpha|S100 alpha|S100 protein, alpha polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100A1, check out the S100A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S100A (Melanoma Marker) S100A1 Monoclonal Antibody (M02503)
Hello CJ!
M02503 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yi M, Yang J, Chen X, Li J, Li X, Wang L, Tan Y, Xiong W, Zhou M, Mccarthy Jb, Li G, Xiang B, Xie H. J Cell Physiol. 2011 Sep;226(9):2360-9. Doi: 10.1002/Jcp.22568. Rassf1A Suppresses Melanoma Development By Modulating Apoptosis And Cell-Cycle Pro...
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Customer Reviews
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Customer Q&As
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Submit your question
5 Customer Q&As for Anti-S100A (Melanoma Marker) S100A1 Monoclonal Antibody
Question
Do you have a BSA free version of anti-S100A (Melanoma Marker) Monoclonal antibody M02503 available?
K. Banerjee
Verified customer
Asked: 2019-10-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100A (Melanoma Marker) Monoclonal antibody M02503 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-10
Question
Does anti-S100A (Melanoma Marker) Monoclonal antibody M02503 work for IHC with caudate nucleus?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
According to the expression profile of caudate nucleus, S100A1 is highly expressed in caudate nucleus. So, it is likely that anti-S100A (Melanoma Marker) Monoclonal antibody M02503 will work for IHC with caudate nucleus.
Boster Scientific Support
Answered: 2019-09-27
Question
I see that the anti-S100A (Melanoma Marker) Monoclonal antibody M02503 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-25
Question
Will M02503 anti-S100A (Melanoma Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
As indicated on the product datasheet, M02503 anti-S100A (Melanoma Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-21
Question
My question regarding product M02503, anti-S100A (Melanoma Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Wu
Verified customer
Asked: 2014-12-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02503 anti-S100A (Melanoma Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-12-25