Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody

S100B antibody

Boster Bio Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody (Catalog # M00979). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow. Cited in 10 publication(s).

Product Info Summary

SKU: M00979
Size: 100ug/vial
Reactive Species: Cow, Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

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Product Name

Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody

View all S100B Antibodies

SKU/Catalog Number

M00979

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody (Catalog # M00979). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00979)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: RS100B/1896

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human S100B protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00979 is reactive to S100B in Cow, Human, Mouse, Rat

Applications

M00979 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

10.713kDa

Background of S100B

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For S100B (Source: Uniprot.org, NCBI)

Gene Name

S100B

Full Name

Protein S100-B

Weight

10.713kDa

Superfamily

S-100 family

Alternative Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta S100B NEF, S100, S100-B, S100beta S100 calcium binding protein B protein S100-B|S-100 calcium-binding protein, beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on S100B, check out the S100B Infographic

S100B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for S100B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00979 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of S100B during the innate immune of corneal epithelium against fungi invasion

Dendritic cells and macrophages neurally hard-wired in the lymph node

Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury

Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection

Pro?neurogenic effects of andrographolide on RSC96 Schwann cells in?vitro

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.

Zhao F, He W, Zhang Y, Tian D, Zhao H, Yu K, Bai J. Neural Regen Res. 2013 Jul 25;8(21):1974-84. Doi: 10.3969/J.Issn.1673-5374.2013.21.006. Electric Stimulation And Decimeter Wave Therapy Improve The Recovery Of Injured Sciatic Nerves.

Qu L, Liang X, Gu B, Liu W. Neural Regen Res. 2014 Jun 15;9(12):1195-203. Doi: 10.4103/1673-5374.135328. Quercetin Alleviates High Glucose-Induced Schwann Cell Damage By Autophagy.

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15 Customer Q&As for Anti-S100B (Astrocyte and Melanoma Marker) Monoclonal Antibody

Question

Would anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 work for IHC with c1 segment of cervical spinal cord?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

According to the expression profile of c1 segment of cervical spinal cord, S100B is highly expressed in c1 segment of cervical spinal cord. So, it is likely that anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 will work for IHC with c1 segment of cervical spinal cord.

Boster Scientific Support

Answered: 2020-04-29

Question

I see that the anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 works with IHC, what is the protocol used to produce the result images on the product page?

Z. Collins

Verified customer

Asked: 2020-04-21

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-21

Question

Can you help my question with product M00979, anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-08

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979. Let me know if you need anything else.

P. Lewis

Verified customer

Asked: 2019-12-12

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-12

Question

Our team were well pleased with the WB result of your anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody. However we have seen positive staining in skin cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

According to literature, skin does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-07

Question

Do you have a BSA free version of anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 available?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-18

Question

Does M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-12

Answer

You can see on the product datasheet, M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-12

Question

See attached the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979. Please let me know if you require anything else.

A. Williams

Verified customer

Asked: 2018-09-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-25

Question

We are currently using anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says bovine, human, rabbit, rat. Is it true that the antibody can work on primate tissues as well?

G. Parker

Verified customer

Asked: 2018-08-09

Answer

The anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody (M00979) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-09

Question

Is a blocking peptide available for product anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody (M00979)?

J. Mangal

Verified customer

Asked: 2018-06-27

Answer

We do provide the blocking peptide for product anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody (M00979). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-27

Question

I was wanting to use your anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody for IHC for bovine c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for bovine c1 segment of cervical spinal cord identification?

Verified Customer

Verified customer

Asked: 2018-02-27

Answer

As indicated on the product datasheet, M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody has been validated for IHC on bovine, human, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-27

Question

Is this M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody reactive to the isotypes of S100B?

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

The immunogen of M00979 anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody is Purified bovine brain S100 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-04

Question

We have observed staining in bovine c1 segment of cervical spinal cord. Any tips? Is anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody supposed to stain c1 segment of cervical spinal cord positively?

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

From what I have seen in literature c1 segment of cervical spinal cord does express S100B. From what I have seen in Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-07-03

Question

I would like to test anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 on bovine c1 segment of cervical spinal cord for research purposes, then I may be interested in using anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Parker

Verified customer

Asked: 2017-01-30

Answer

The products we sell, including anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-01-30

Question

Would anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 work on canine IHC with skin?

K. Huang

Verified customer

Asked: 2013-01-16

Answer

Our lab technicians have not tested anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 on canine. You can run a BLAST between canine and the immunogen sequence of anti-S100B (Astrocyte and Melanoma Marker) Monoclonal antibody M00979 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine skin in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-01-16

Order DetailsPrice
M00979-100ug

100 μg

$468

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