Anti-SARS-CoV-2 NSP12/RNA polymerase Antibody

Boster Bio Anti-SARS-CoV-2 NSP12/RNA polymerase Antibody catalog # A34004. Tested in ELISA applications. This antibody reacts with Human.

Product Info Summary

SKU: A34004
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA

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Product Name

Anti-SARS-CoV-2 NSP12/RNA polymerase Antibody

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SKU/Catalog Number

A34004

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SARS-CoV-2 NSP12/RNA polymerase Antibody catalog # A34004. Tested in ELISA applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SARS-CoV-2 NSP12/RNA polymerase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A34004)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Clone Number

IC-16

Isotype

Rabbit IgG

Immunogen

SADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKggggDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A34004 is reactive to in Human

Applications

A34004 is guaranteed for ELISA Boster Guarantee

Observed Molecular Weight

140 kDa, 130 kDa, 110 kDa

Calculated molecular weight

16693 MW

Background of

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA, 0.001-0.1μg/ml, Human

Validation Images & Assay Conditions

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Order DetailsPrice
A34004

100μg

$415
A34004-10ug

10μg sample (liquid)

$99
A34004-Biotin

100 μg Biotin conjugated

$615
A34004-Cy3

100 μg Cy3 conjugated

$615
A34004-Dylight488

100 μg Dylight488 conjugated

$615
A34004-Dylight550

100 μg Dylight550 conjugated

$615
A34004-Dylight594

100 μg Dylight594 conjugated

$615
A34004-FITC

100 μg FITC conjugated

$615
A34004-HRP

100 μg HRP conjugated

$615
A34004-APC

100 μg APC conjugated

$715
A34004-PE

100 μg PE conjugated

$715
A34004-carrier-free

Carrier Free

$415

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A34004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$415.00

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