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SKU:RP1035
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-SCP3 Antibody
SKU/Catalog Number RP1035
Description Rabbit IgG polyclonal antibody for Synaptonemal complex protein 3(SYCP3) detection. Tested with WB in Human.
Cite This Product Anti-SCP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1035)
Replacement Item This antibody may replace the following items: sc-33875 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human SCP3 recombinant protein (Position: E18-E223). Human SCP3 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat SCP3, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SYCP3
Protein Name Synaptonemal complex protein 3
Molecular Weight 27729 MW
Protein Function Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity). .
Tissue Specificity Testis-specific. .
Subcellular Localization Nucleus . Chromosome . In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. .
Uniprot ID Q8IZU3
Alternative Names Synaptonemal complex protein 3;SCP-3;SYCP3;SCP3;
Research Areas SYCP3|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Synaptonemal complex protein 3

SYCP3, also known as COR1 or SCP3, is a protein that in humans is encoded by the SYCP3 gene. It is mapped to 12q23.2. This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. SYCP3 has an essential meiotic function in human spermatogenesis that is compromised by the mutant protein by dominant-negative interference. SYCP3 is linked to inherited aneuploidy in female germ cells and provides a model system for studying age-dependent degeneration in oocytes.

Anti-SCP3 Antibody Images

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Anti-SCP3 Antibody
Anti-SCP3 Picoband antibody, RP1035-1.jpg
All lanes: Anti SCP3 (RP1035) at 0.5ug/ml
WB: Recombinant Human SCP3 Protein 0.5ng
Predicted bind size: 41KD
Observed bind size: 41KD
Anti-SCP3 Antibody
Anti-SCP3 Picoband antibody, RP1035-2.jpg
All lanes: Anti SCP3 (RP1035) at 0.5ug/ml
WB: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to scp3 antibody, sycp3 antibody