Anti-Securin/PTTG1 Antibody

PTTG1 antibody

Boster Bio Anti-Securin/PTTG1 Antibody catalog # PA2240. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PA2240
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Securin/PTTG1 Antibody

View all PTTG1 Antibodies

SKU/Catalog Number

PA2240

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Securin/PTTG1 Antibody catalog # PA2240. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Securin/PTTG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2240)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Securin, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2240 is reactive to PTTG1 in Human

Applications

PA2240 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

22.024kDa

Background of PTTG1

Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PTTG1 (Source: Uniprot.org, NCBI)

Gene Name

PTTG1

Full Name

Securin

Weight

22.024kDa

Superfamily

securin family

Alternative Names

EAP1MGC126883; ESP1-associated protein 1; Esp1-associated protein; hPTTG; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; PTTGMGC138276; securin; Tumor-transforming protein 1; TUTR1HPTTG PTTG1 EAP1, HPTTG, PTTG, TUTR1 PTTG1 regulator of sister chromatid separation, securin securin|ESP1-associated protein 1|pituitary tumor-transforming 1|pituitary tumor-transforming gene 1 protein|tumor-transforming protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTTG1, check out the PTTG1 Infographic

PTTG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTTG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2240 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang E, Liu S, Xu Z, Huang S, Tan X, Sun C, Lu L. Tumour Biol. 2014 Sep;35(9):8801-11. Doi: 10.1007/S13277-014-2143-2. Epub 2014 May 31. Pituitary Tumor-Transforming Gene 1 (Pttg1) Is Overexpressed In Oral Squamous Cell Carcinoma (Oscc) And Promo...

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1 Customer Q&As for Anti-Securin/PTTG1 Antibody

Question

We are currently using anti-Securin/PTTG1 antibody PA2240 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

The anti-Securin/PTTG1 antibody (PA2240) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-27

Order DetailsPrice
PA2240

100μg

$370
PA2240-10ug

10μg sample (liquid)

$99
PA2240-Biotin

100 μg Biotin conjugated

$570
PA2240-Cy3

100 μg Cy3 conjugated

$570
PA2240-Dylight488

100 μg Dylight488 conjugated

$570
PA2240-Dylight550

100 μg Dylight550 conjugated

$570
PA2240-Dylight594

100 μg Dylight594 conjugated

$570
PA2240-FITC

100 μg FITC conjugated

$570
PA2240-HRP

100 μg HRP conjugated

$570
PA2240-APC

100 μg APC conjugated

$670
PA2240-PE

100 μg PE conjugated

$670
PA2240-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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