Product Info Summary
SKU: | PA2240 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Securin/PTTG1 Antibody
SKU/Catalog Number
PA2240
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Securin/PTTG1 Antibody catalog # PA2240. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Securin/PTTG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2240)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Securin, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2240 is reactive to PTTG1 in Human
Applications
PA2240 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.024kDa
Background of PTTG1
Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Securin antibody, PA2240, Western blotting
All lanes: Anti PTTG1 (PA2240) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: A375 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
Protein Target Info & Infographic
Gene/Protein Information For PTTG1 (Source: Uniprot.org, NCBI)
Gene Name
PTTG1
Full Name
Securin
Weight
22.024kDa
Superfamily
securin family
Alternative Names
EAP1MGC126883; ESP1-associated protein 1; Esp1-associated protein; hPTTG; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; PTTGMGC138276; securin; Tumor-transforming protein 1; TUTR1HPTTG PTTG1 EAP1, HPTTG, PTTG, TUTR1 PTTG1 regulator of sister chromatid separation, securin securin|ESP1-associated protein 1|pituitary tumor-transforming 1|pituitary tumor-transforming gene 1 protein|tumor-transforming protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTTG1, check out the PTTG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTTG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Securin/PTTG1 Antibody (PA2240)
Hello CJ!
PA2240 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang E, Liu S, Xu Z, Huang S, Tan X, Sun C, Lu L. Tumour Biol. 2014 Sep;35(9):8801-11. Doi: 10.1007/S13277-014-2143-2. Epub 2014 May 31. Pituitary Tumor-Transforming Gene 1 (Pttg1) Is Overexpressed In Oral Squamous Cell Carcinoma (Oscc) And Promo...
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Securin/PTTG1 Antibody
Question
We are currently using anti-Securin/PTTG1 antibody PA2240 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-Securin/PTTG1 antibody (PA2240) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27