Show Order Info
SKU:PA1720
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-SERCA2 ATPase Antibody
SKU/Catalog Number PA1720
Description Rabbit IgG polyclonal antibody for Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(ATP2A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-SERCA2 ATPase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1720)
Replacement Item This antibody may replace the following items: sc-271669|sc-30110|sc-376235|sc-53010|sc-73022|sc-8094|sc-8095 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase(665-679aa QRDACLNARCFARVE), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ATP2A2
Protein Name Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(SERCA2/SR Ca(2+)-ATPase 2)
Molecular Weight 114757 MW
Protein Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. .
Tissue Specificity Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .
Sequence Similarities Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Subcellular Localization Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Uniprot ID P16615
Alternative Names Sarcoplasmic/endoplasmic reticulum calcium ATPase 2;SERCA2;SR Ca(2+)-ATPase 2;3.6.3.8;Calcium pump 2;Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca(2+) ATPase;ATP2A2;ATP2B;
Research Areas |signal transduction|signaling pathway|calcium signaling|calcium channels| signal transduction|metabolism|plasma membrane|atpases|vitamins / minerals| cardiovascular|heart|contractility|inotropics| metabolism|pathways and processes|cofactors, vitamins / minerals|types of disease|cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(SERCA2/SR Ca(2+)-ATPase 2)

SERCA2(SARCOPLASMIC RETICULUM Ca(2+)-ATPase 2), also called ATP2A2, ATP2B, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca(2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped on 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression.SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells. Perilesional Darier disease skin was comparable to normal skin.

Anti-SERCA2 ATPase Antibody Images

Click the images to enlarge.

Anti-SERCA2 ATPase Antibody
Anti-SERCA2 ATPase antibody, PA1720, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-SERCA2 ATPase Antibody
Anti-SERCA2 ATPase antibody, PA1720, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate

Customer Reviews

Review by
Rating:

Source: Biocompare.com

"Good Antibody for Western Blotting"

--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE

Applications Western Blot
Sample MDCK cell lysate
Detection ECL

"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."

(Posted on 8/23/2016)
Write a review for PA1720

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to serca2 antibody, serca2a antibody