|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-SERCA2 ATPase Antibody|
|Description||Rabbit IgG polyclonal antibody for Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(ATP2A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SERCA2 ATPase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1720)|
|Replacement Item||This antibody may replace the following items: sc-271669|sc-30110|sc-376235|sc-53010|sc-73022|sc-8094|sc-8095 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase(665-679aa QRDACLNARCFARVE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(SERCA2/SR Ca(2+)-ATPase 2)|
|Molecular Weight||114757 MW|
|Protein Function||This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. .|
|Tissue Specificity||Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. .|
|Sequence Similarities||Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.|
|Subcellular Localization||Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.|
|Alternative Names||Sarcoplasmic/endoplasmic reticulum calcium ATPase 2;SERCA2;SR Ca(2+)-ATPase 2;22.214.171.124;Calcium pump 2;Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca(2+) ATPase;ATP2A2;ATP2B;|
|Research Areas|||signal transduction|signaling pathway|calcium signaling|calcium channels| signal transduction|metabolism|plasma membrane|atpases|vitamins / minerals| cardiovascular|heart|contractility|inotropics| metabolism|pathways and processes|cofactors, vitamins / minerals|types of disease|cancer||
Background for Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(SERCA2/SR Ca(2+)-ATPase 2)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SERCA2 ATPase Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SERCA2 ATPase Antibody Images
Click the images to enlarge.
IHC(P): Human Mammary Cancer Tissue
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate
- Review by Sona Balasubramaniam
"Good Antibody for Western Blotting"
--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE
Applications Western Blot Sample MDCK cell lysate Detection ECL
"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,