Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody

SLC6A4/5-HTTLPR/Serotonin transporter antibody

Boster Bio Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody (Catalog # P00496). Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: P00496
Size: 100 µl
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody

View all SLC6A4/5-HTTLPR/Serotonin transporter Antibodies

SKU/Catalog Number

P00496

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody (Catalog # P00496). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00496)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 of rat SERT, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00496 is reactive to SLC6A4 in Mouse, Rat

Applications

P00496 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

70.325kDa

Background of SLC6A4/5-HTTLPR/Serotonin transporter

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at Thr-276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at Thr-276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the Thr-276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For SLC6A4 (Source: Uniprot.org, NCBI)

Gene Name

SLC6A4

Full Name

Sodium-dependent serotonin transporter

Weight

70.325kDa

Superfamily

sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family

Alternative Names

5HT transporter; 5-HT Transporter; 5-HTT; 5HTTLPR; 5-HTTLPR; hSERT; HTT5-hydroxytryptamine transporter; Na+/Cl- dependent serotonin transporter; OCD1; Serotonin Transporter 1; SERT; SLC6A4; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4,5-HTTLPR; Solute carrier family 6 member 4,5HTT SLC6A4 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT solute carrier family 6 member 4 sodium-dependent serotonin transporter|5-hydroxytryptamine (serotonin) transporter|5HT transporter|Na+/Cl- dependent serotonin transporter|serotonin transporter 1|solute carrier family 6 (neurotransmitter transporter), member 4|solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC6A4, check out the SLC6A4 Infographic

SLC6A4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC6A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Phospho-Serotonin Transporter (Thr276) SLC6A4 Antibody

Question

My question regards to test anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 on mouse brain for research purposes, then I may be interested in using anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

The products we sell, including anti-Phospho-Serotonin Transporter (Thr276) antibody P00496, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-30

Question

Will anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 work for WB with brain?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

According to the expression profile of brain, SLC6A4 is highly expressed in brain. So, it is likely that anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 will work for WB with brain.

Boster Scientific Support

Answered: 2019-10-09

Question

I was wanting to use your anti-Phospho-Serotonin Transporter (Thr276) antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

It shows on the product datasheet, P00496 anti-Phospho-Serotonin Transporter (Thr276) antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-04

Question

Is there a BSA free version of anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 available?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-15

Question

I have attached the WB image, lot number and protocol we used for brain using anti-Phospho-Serotonin Transporter (Thr276) antibody P00496. Please let me know if you require anything else.

W. Taylor

Verified customer

Asked: 2018-05-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-02

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Phospho-Serotonin Transporter (Thr276) antibody P00496. Let me know if you need anything else.

T. Patel

Verified customer

Asked: 2015-12-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-28

Question

Does anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 work on canine WB with brain?

S. Banerjee

Verified customer

Asked: 2014-02-12

Answer

Our lab technicians have not tested anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Phospho-Serotonin Transporter (Thr276) antibody P00496 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-02-12

Order DetailsPrice
P00496

100uL

$465

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