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SKU:PB9021
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-SAP/PTX2 Antibody
SKU/Catalog Number PB9021
Description Rabbit IgG polyclonal antibody for Serum amyloid P-component(APCS) detection. Tested with WB, IHC-P in Rat.
Cite This Product Anti-SAP/PTX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9021)
Replacement Item This antibody may replace the following items: sc-100528|sc-135069|sc-138078|sc-138079|sc-15803|sc-15804|sc-163950|sc-18309|sc-18310|sc-245912|sc-245913|sc-246555|sc-246556|sc-246557|sc-292686|sc-292687|sc-292972|sc-368736|sc-374654|sc-377175|sc-393704|sc-393948|sc-52718|sc-52719|sc-53165|sc-53166|sc-53647|sc-54538|sc-54539|sc-59652|sc-65398|sc-65958|sc-65959|sc-69662|sc-69796|sc-71094|sc-7930|sc-80504|sc-81682|sc-81683|sc-82714|sc-84112|sc-84113|sc-895|sc-896|sc-9998 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived rat Serum Amyloid P recombinant protein (Position: Q21-S228). Rat Serum Amyloid P shares 72% and 80% amino acid (aa) sequences identity with human and mouse Serum Amyloid P, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Apcs
Protein Name Serum amyloid P-component
Molecular Weight 26176 MW
Sequence Similarities Belongs to the pentaxin family.
Subcellular Localization Secreted.
Uniprot ID P23680
Alternative Names Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;
Research Areas |kits/ lysates/ other|elisa kits|neuroscience target elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Serum amyloid P-component

Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Anti-SAP/PTX2 Antibody Images

Click the images to enlarge.

Anti-SAP/PTX2 Antibody
Anti-Serum Amyloid P Picoband antibody, PB9021-1.JPG
IHC(P): Rat Liver Tissue
Anti-SAP/PTX2 Antibody
Anti-Serum Amyloid P Picoband antibody, PB9021-2.jpg
All lanes: Anti- Serum Amyloid P(PB9021) at 0.5ug/ml
WB: Recombinant Rat Serum Amyloid P Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is sap antibody