Anti-SFPQ Monoclonal Antibody

SFPQ antibody

Boster Bio Anti-SFPQ Monoclonal Antibody catalog # M02243. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02243
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-SFPQ Monoclonal Antibody

View all SFPQ Antibodies

SKU/Catalog Number

M02243

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SFPQ Monoclonal Antibody catalog # M02243. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SFPQ Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02243)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AECI-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SFPQ DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02243 is reactive to SFPQ in Human, Mouse, Rat

Applications

M02243 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

116 kDa

Calculated molecular weight

76.149kDa

Background of SFPQ

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For SFPQ (Source: Uniprot.org, NCBI)

Gene Name

SFPQ

Full Name

Splicing factor, proline- and glutamine-rich

Weight

76.149kDa

Alternative Names

100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; hPOMp100; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; PSFPOMP100; PTB-associated splicing factor; PTB-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated); splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated); splicing factor proline/glutamine-rich; splicing factor, proline- and glutamine-r SFPQ POMP100, PPP1R140, PSF splicing factor proline and glutamine rich splicing factor, proline- and glutamine-rich|100 kDa DNA-pairing protein|DNA-binding p52/p100 complex, 100 kDa subunit|PTB-associated splicing factor|epididymis secretory sperm binding protein|polypyrimidine tract binding protein associated|polypyrimidine tract-binding protein-associated splicing factor|protein phosphatase 1, regulatory subunit 140|splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)|splicing factor proline/glutamine-rich

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SFPQ, check out the SFPQ Infographic

SFPQ infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SFPQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-SFPQ Monoclonal Antibody

Question

We are currently using anti-SFPQ Monoclonal antibody M02243 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

The anti-SFPQ Monoclonal antibody (M02243) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-05-05

Question

I was wanting to use your anti-SFPQ Monoclonal antibody for IF for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

It shows on the product datasheet, M02243 anti-SFPQ Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-15

Question

What sample was tested in WB and IHC applications with M02243?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of SFPQ expression in A431 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

Will anti-SFPQ Monoclonal antibody M02243 work for IF with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

According to the expression profile of leukemic t-cell, SFPQ is highly expressed in leukemic t-cell. So, it is likely that anti-SFPQ Monoclonal antibody M02243 will work for IF with leukemic t-cell.

Boster Scientific Support

Answered: 2019-06-03

Order DetailsPrice
M02243

100μL

$370
M02243-30ul

30μL sample

$99
M02243-carrier-free

Carrier Free

$370

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