Anti-SGK269 PEAK1 Antibody

PEAK1 antibody

Boster Bio Anti-SGK269 PEAK1 Antibody (Catalog # A06989). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A06989
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-SGK269 PEAK1 Antibody

View all PEAK1 Antibodies

SKU/Catalog Number

A06989

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SGK269 PEAK1 Antibody (Catalog # A06989). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SGK269 PEAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06989)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human SGK269.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06989 is reactive to PEAK1 in Human, Mouse

Applications

A06989 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

193.106kDa

Background of PEAK1

Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.

Zody M.C., Nature 440:671-675(2006).
Jikuya H., Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Ohara O., DNA Res. 9:47-57(2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For PEAK1 (Source: Uniprot.org, NCBI)

Gene Name

PEAK1

Full Name

Inactive tyrosine-protein kinase PEAK1

Weight

193.106kDa

Superfamily

protein kinase superfamily

Alternative Names

FLJ21140; FLJ34483; KIAA2002; NKF3 kinase family member; pseudopodium enriched atypical kinase; pseudopodium-enriched atypical kinase 1; SGK269; sugen kinase 269; tyrosine-protein kinase SgK269 PEAK1 SGK269 pseudopodium enriched atypical kinase 1 inactive tyrosine-protein kinase PEAK1|NKF3 kinase family member|sugen kinase 269|tyrosine-protein kinase SgK269

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PEAK1, check out the PEAK1 Infographic

PEAK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PEAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-SGK269 PEAK1 Antibody

Question

We are currently using anti-SGK269 antibody A06989 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

The anti-SGK269 antibody (A06989) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-08

Question

See below the WB image, lot number and protocol we used for cervix carcinoma using anti-SGK269 antibody A06989. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-23

Question

My question regarding product A06989, anti-SGK269 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06989 anti-SGK269 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-03-21

Question

Do you have a BSA free version of anti-SGK269 antibody A06989 available?

Verified Customer

Verified customer

Asked: 2018-03-22

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SGK269 antibody A06989 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-22

Question

Will anti-SGK269 antibody A06989 work for WB with cervix carcinoma?

S. Yang

Verified customer

Asked: 2018-01-29

Answer

According to the expression profile of cervix carcinoma, PEAK1 is highly expressed in cervix carcinoma. So, it is likely that anti-SGK269 antibody A06989 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2018-01-29

Question

Will anti-SGK269 antibody A06989 work on horse WB with mesangial cell smooth muscle?

Verified Customer

Verified customer

Asked: 2017-10-30

Answer

Our lab technicians have not validated anti-SGK269 antibody A06989 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SGK269 antibody A06989 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse mesangial cell smooth muscle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-30

Question

I was wanting to use your anti-SGK269 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?

G. Baker

Verified customer

Asked: 2015-03-27

Answer

It shows on the product datasheet, A06989 anti-SGK269 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-03-27

Order DetailsPrice
A06989-100uL

100uL

$360

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