Anti-SHC/SHC1 Antibody

SHC1 antibody

Boster Bio Anti-SHC/SHC1 Antibody catalog # PA1897. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1897
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, IHC-F, ICC, WB

Product Name

Anti-SHC/SHC1 Antibody

View all SHC1 Antibodies

SKU/Catalog Number

PA1897

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SHC/SHC1 Antibody catalog # PA1897. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SHC/SHC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1897)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1897 is reactive to SHC1 in Human, Mouse, Rat

Applications

PA1897 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

46 kDa, 52 kDa, 66 kDa

Calculated molecular weight

62.822kDa

Background of SHC1

SHC1 (SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al. (1994) assigned the SHC1 gene to 1q21. Yulug et al. (1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence (SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al. (1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel (2002) concluded that there is an important functional relationship between forkhead proteins (e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For SHC1 (Source: Uniprot.org, NCBI)

Gene Name

SHC1

Full Name

SHC-transforming protein 1

Weight

62.822kDa

Alternative Names

FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC (Src homology 2 domain-containing) transforming protein 1; SHC; SHC1; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1 SHC1 SHC, SHCA SHC adaptor protein 1 SHC-transforming protein 1|SH2 domain protein C1|SHC (Src homology 2 domain containing) transforming protein 1|SHC-transforming protein 3|SHC-transforming protein A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SHC1, check out the SHC1 Infographic

SHC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SHC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-SHC/SHC1 Antibody

Question

Is a blocking peptide available for product anti-SHC/SHC1 antibody (PA1897)?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

We do provide the blocking peptide for product anti-SHC/SHC1 antibody (PA1897). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-06

Question

Has PA1897 detected 3 isoforms in any of your tested samples?

Verified customer

Asked: 2019-04-03

Answer

The Anti-SHC/SHC1 Antibody (PA1897) is able to detect 3 isoforms.

Boster Scientific Support

Answered: 2019-04-15

Question

Would anti-SHC/SHC1 antibody PA1897 work for WB with choriocarcinoma neuroblastoma?

Verified Customer

Verified customer

Asked: 2019-02-26

Answer

According to the expression profile of choriocarcinoma neuroblastoma, SHC1 is highly expressed in choriocarcinoma neuroblastoma. So, it is likely that anti-SHC/SHC1 antibody PA1897 will work for WB with choriocarcinoma neuroblastoma.

Boster Scientific Support

Answered: 2019-02-26

Question

I have a question about product PA1897, anti-SHC/SHC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Brown

Verified customer

Asked: 2018-12-03

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1897 anti-SHC/SHC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for choriocarcinoma neuroblastoma using anti-SHC/SHC1 antibody PA1897. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-06-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-02

Order DetailsPrice
PA1897

100μg

$370
PA1897-10ug

10μg sample (liquid)

$99
PA1897-Biotin

100 μg Biotin conjugated

$570
PA1897-Cy3

100 μg Cy3 conjugated

$570
PA1897-Dylight488

100 μg Dylight488 conjugated

$570
PA1897-Dylight550

100 μg Dylight550 conjugated

$570
PA1897-Dylight594

100 μg Dylight594 conjugated

$570
PA1897-FITC

100 μg FITC conjugated

$570
PA1897-HRP

100 μg HRP conjugated

$570
PA1897-APC

100 μg APC conjugated

$670
PA1897-PE

100 μg PE conjugated

$670
PA1897-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1897
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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