|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-SHC Antibody|
|Description||Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-SHC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1897)|
|Replacement Item||This antibody may replace the following items: sc-101796|sc-101797|sc-1695|sc-18074-R|sc-18075|sc-23764|sc-23765-R|sc-288|sc-293148|sc-393717|sc-398163|sc-398289|sc-81518|sc-81519|sc-81520|sc-967 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SHC(424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||SHC-transforming protein 1|
|Molecular Weight||62822 MW|
|Protein Function||Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. .|
|Tissue Specificity||Widely expressed. Expressed in neural stem cells but absent in mature neurons.|
|Sequence Similarities||Contains 1 PID domain.|
|Alternative Names||SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1;SHC1;SHC, SHCA;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|amyloid| signal transduction|adapters|cytoplasmic||
Background for SHC-transforming protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SHC Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SHC Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD (162aa tag+D424-P578)
Lane 1: Recombinant Human SHC1 Proteins 10ng
Lane 2: Recombinant Human SHC1 Proteins 5ng
Lane 3: Recombinant Human SHC1 Proteins 2.5ng
Lane 1: Rat Brain Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: 293T Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
IHC(P): Rat Brain Tissue
IHC(P): Human Lung Cancer Tissue
IHC(F): Rat Intestine Tissue
IHC(F): Rat Brain Tissue
ICC: A549 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,