Anti-SHP2/PTPN11 Antibody

SHP-2/PTPN11 antibody

Boster Bio Anti-SHP2/PTPN11 Antibody catalog # PA1860. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1860
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-SHP2/PTPN11 Antibody

View all SHP-2/PTPN11 Antibodies

SKU/Catalog Number

PA1860

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SHP2/PTPN11 Antibody catalog # PA1860. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SHP2/PTPN11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1860)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHP2, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1860 is reactive to PTPN11 in Human, Mouse, Rat

Applications

PA1860 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

68.011kDa

Background of SHP-2/PTPN11

PTPN11 (Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D (PTP-1D), protein-tyrosine phosphatase 2C (PTP-2C), TYROSINE PHOSPHATASE SHP2 (SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an enzyme that in humans is encoded by the PTPN11 gene. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For PTPN11 (Source: Uniprot.org, NCBI)

Gene Name

PTPN11

Full Name

Tyrosine-protein phosphatase non-receptor type 11

Weight

68.011kDa

Superfamily

protein-tyrosine phosphatase family

Alternative Names

BPTP3; EC 3.1.3.48; MGC14433; protein tyrosine phosphatase, non-receptor type 11; protein tyrosine phosphatase-2; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP1D; PTP-1D; PTP2C; PTP-2C; PTP2CNS1; PTPN11; SHP2; SHP-2; SHP2CFC; SHPTP2; SH-PTP2; SH-PTP2Noonan syndrome 1; SH-PTP3; tyrosine-protein phosphatase non-receptor type 11 PTPN11 BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 protein tyrosine phosphatase non-receptor type 11 tyrosine-protein phosphatase non-receptor type 11|SH2 domain-containing protein tyrosine phosphatase 2|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTPN11, check out the PTPN11 Infographic

PTPN11 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTPN11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-SHP2/PTPN11 Antibody

Question

Would PA1860 anti-SHP2/PTPN11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

You can see on the product datasheet, PA1860 anti-SHP2/PTPN11 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-05

Question

you antibody to test anti-SHP2/PTPN11 antibody PA1860 on human leukemic t-cell for research purposes, then I may be interested in using anti-SHP2/PTPN11 antibody PA1860 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-20

Answer

The products we sell, including anti-SHP2/PTPN11 antibody PA1860, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-20

Question

Does anti-SHP2/PTPN11 antibody PA1860 work for WB with leukemic t-cell?

K. Lewis

Verified customer

Asked: 2015-06-03

Answer

According to the expression profile of leukemic t-cell, PTPN11 is highly expressed in leukemic t-cell. So, it is likely that anti-SHP2/PTPN11 antibody PA1860 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2015-06-03

Order DetailsPrice
PA1860

100μg

$370
PA1860-10ug

10μg sample (liquid)

$99
PA1860-Biotin

100 μg Biotin conjugated

$570
PA1860-Cy3

100 μg Cy3 conjugated

$570
PA1860-Dylight488

100 μg Dylight488 conjugated

$570
PA1860-Dylight550

100 μg Dylight550 conjugated

$570
PA1860-Dylight594

100 μg Dylight594 conjugated

$570
PA1860-FITC

100 μg FITC conjugated

$570
PA1860-HRP

100 μg HRP conjugated

$570
PA1860-APC

100 μg APC conjugated

$670
PA1860-PE

100 μg PE conjugated

$670
PA1860-carrier-free

Carrier Free

$370

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