|Sample Size:||30ug for $99, contact us for details|
|Application:||Flow Cytometry, IHC, ICC, WB|
Data & Images
|Product Name||Anti-SHP2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Tyrosine-protein phosphatase non-receptor type 11(PTPN11) detection. Tested with WB, IHC-F, ICC, FCM in Human;Mouse;Rat.|
|Cite This Product||Anti-SHP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1860)|
|Replacement Item||This antibody may replace the following items: sc-101798|sc-101799|sc-12737|sc-130606|sc-136271|sc-17750|sc-271053|sc-271106|sc-271550|sc-280|sc-293147|sc-30687|sc-30688|sc-30689|sc-365447|sc-365488|sc-424|sc-55507|sc-58301|sc-58304|sc-7384|sc-7568|sc-7569|sc-9116 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||Flow Cytometry, IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SHP2(582-597aa RVYENVGLMQQQKSFR), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tyrosine-protein phosphatase non-receptor type 11|
|Molecular Weight||68436 MW|
|Protein Function||Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. .|
|Tissue Specificity||Widely expressed, with highest levels in heart, brain, and skeletal muscle. .|
|Sequence Similarities||Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.|
|Alternative Names||Tyrosine-protein phosphatase non-receptor type 11;184.108.40.206;Protein-tyrosine phosphatase 1D;PTP-1D;Protein-tyrosine phosphatase 2C;PTP-2C;SH-PTP2;SHP-2;Shp2;SH-PTP3;PTPN11;PTP2C, SHPTP2;|
|Research Areas|||signal transduction|protein phosphorylation|tyrosine phosphatases| neuroscience|sensory system|auditory system||
Background for Tyrosine-protein phosphatase non-receptor type 11
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SHP2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SHP2 Antibody Images
Click the images to enlarge.
All lanes: Anti SHP2 (PA1860) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
LAll lanes: Anti SHP2 (PA1860) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Heart Tissue Lysate at 50ug
Lane 4: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: U87 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,