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SKU:PB9161
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-SIRT3/Sirtuin 3 Picoband™ Antibody
SKU/Catalog Number PB9161
Description Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-3, mitochondrial(SIRT3) detection. Tested with WB in Human;Rat.
Cite This Product Anti-SIRT3/Sirtuin 3 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9161)
Replacement Item This antibody may replace the following items: sc-49744|sc-49743|sc-99143|sc-365175|sc-135796|sc-135795 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SIRT3
Protein Name NAD-dependent protein deacetylase sirtuin-3, mitochondrial
Molecular Weight 43573 MW
Protein Function NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. .
Tissue Specificity Widely expressed. .
Sequence Similarities Belongs to the sirtuin family. Class I subfamily.
Subcellular Localization Mitochondrion matrix .
Uniprot ID Q9NTG7
Alternative Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial;hSIRT3;3.5.1.-;Regulatory protein SIR2 homolog 3;SIR2-like protein 3;SIRT3;SIR2L3;
Research Areas |epigenetics and nuclear signaling|chromatin modifying enzymes|acetylation| cell biology|cell cycle|cell division|other cell division antibodies| epigenetics and nuclear signaling|hdacs|class iii / sir2 class| cancer|oncoproteins/suppressors|tumor suppressors| cardiovascular|heart|cardiac metabolism| metabolism|types of disease|obesity|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for NAD-dependent protein deacetylase sirtuin-3, mitochondrial

NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

Anti-SIRT3/Sirtuin 3 Picoband™ Antibody Images

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Anti-SIRT3/Sirtuin 3 Picoband™ Antibody
Anti-SIRT3 Picoband antibody, PB9161-1.jpg
All lanes: Anti SIRT3 (PB9161) at 0.5ug/ml
WB: Recombinant Human SIRT3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-SIRT3/Sirtuin 3 Picoband™ Antibody
Anti-SIRT3 Picoband antibody, PB9161-2.jpg
All lanes: Anti SIRT3 (PB9161) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to sirt3 antibody, sirtuin 3 antibody