|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-SLC2A1/Glut1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 1(SLC2A1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-SLC2A1/Glut1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1120-1)|
|Replacement Item||This antibody may replace the following items: sc-79103|sc-79102|sc-79100|sc-98362|sc-377405|sc-81383|sc-514232 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A1(477-492aa KTPEELFHPLGADSQV), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Solute carrier family 2, facilitated glucose transporter member 1|
|Molecular Weight||54084 MW|
|Protein Function||Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. .|
|Tissue Specificity||Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues. .|
|Sequence Similarities||Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Melanosome. Localizes primarily at the cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Alternative Names||Solute carrier family 2, facilitated glucose transporter member 1;Glucose transporter type 1, erythrocyte/brain;GLUT-1;HepG2 glucose transporter;SLC2A1;GLUT1;|
|Research Areas|||signal transduction|metabolism|energy metabolism| signal transduction|plasma membrane|channels| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways||
Background for Solute carrier family 2, facilitated glucose transporter member 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SLC2A1/Glut1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SLC2A1/Glut1 Antibody Images
Click the images to enlarge.
All lanes: Anti SLC2A1(PA1120-1) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,