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SKU:PA2064
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-SLC2A5/Glut5 Antibody
SKU/Catalog Number PA2064
Description Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 5(SLC2A5) detection. Tested with WB in Human.
Cite This Product Anti-SLC2A5/Glut5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2064)
Replacement Item This antibody may replace the following items: sc-14841|sc-271055|sc-30109|sc-31841|sc-31844 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human SLC2A5 (481-501aa VSEVYPEKEELKELPPVTSEQ).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SLC2A5
Protein Name Solute carrier family 2, facilitated glucose transporter member 5
Molecular Weight 54974 MW
Protein Function Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
Tissue Specificity Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
Sequence Similarities Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Subcellular Localization Apical cell membrane ; Multi- pass membrane protein . Membrane; Multi-pass membrane protein. Localized on the apical membrane of the small intestine and the proximal tubule of the kidney. .
Uniprot ID P22732
Alternative Names Solute carrier family 2, facilitated glucose transporter member 5;Fructose transporter;Glucose transporter type 5, small intestine;GLUT-5;SLC2A5;GLUT5;
Research Areas |signal transduction|metabolism|energy metabolism| signal transduction|plasma membrane|channels| cancer|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates| metabolism|pathways and processes|metabolic signaling pathways|carbohydrate metabolism|energy transfer pathways|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Solute carrier family 2, facilitated glucose transporter member 5

SLC2A5, also known as GLUT5(Glucose transporter 5), is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient. In humans the GLUT5 protein is encoded by the SLC2A5 gene.

Anti-SLC2A5/Glut5 Antibody Images

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Anti-SLC2A5/Glut5 Antibody
Anti-SLC2A5 antibody, PA2064, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: 293T Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: SW620 Cell Lysate
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.