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SKU:PB9151
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-SLC9A1/Nhe1 Picoband™ Antibody
SKU/Catalog Number PB9151
Description Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 1(SLC9A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-SLC9A1/Nhe1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9151)
Replacement Item This antibody may replace the following items: sc-70164|sc-98566|sc-166809|sc-166810|sc-135868 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SLC9A1
Protein Name Sodium/hydrogen exchanger 1
Molecular Weight 90763 MW
Protein Function Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. .
Tissue Specificity Kidney and intestine.
Sequence Similarities Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Subcellular Localization Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane. .
Uniprot ID P19634
Alternative Names Sodium/hydrogen exchanger 1;APNH;Na(+)/H(+) antiporter, amiloride-sensitive;Na(+)/H(+) exchanger 1;NHE-1;Solute carrier family 9 member 1;SLC9A1;APNH1, NHE1;
Research Areas |signal transduction|adapters|transmembrane| signal transduction|metabolism|plasma membrane|channels| cardiovascular|atherosclerosis|hypertension|sodium/ion transport| metabolism|types of disease|cancer|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Sodium/hydrogen exchanger 1

NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Anti-SLC9A1/Nhe1 Picoband™ Antibody Images

Click the images to enlarge.

Anti-SLC9A1/Nhe1 Picoband™ Antibody
Anti-SLC9A1 Picoband antibody, PB9151-1.jpg
All lanes: Anti SLC9A1 (PB9151) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-SLC9A1/Nhe1 Picoband™ Antibody
Anti-SLC9A1 Picoband antibody, PB9151-2.jpg
All lanes: Anti SLC9A1 (PB9151) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
Anti-SLC9A1/Nhe1 Picoband™ Antibody
Anti-SLC9A1 Picoband antibody, PB9151-3.JPG
IHC(P): Rat Kidney Tissue
Anti-SLC9A1/Nhe1 Picoband™ Antibody
Anti-SLC9A1 Picoband antibody, PB9151-4.JPG
IHC(P): Human Mammary Cancer Tissue
Anti-SLC9A1/Nhe1 Picoband™ Antibody
Anti-SLC9A1 Picoband antibody, PB9151-5.JPG
IHC(P): Mouse Kidney Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nhe1 antibody, nhe-1 antibody