Data & Images
|Product Name||Anti-SLC9A1/Nhe1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 1(SLC9A1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SLC9A1/Nhe1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9151)|
|Replacement Item||This antibody may replace the following items: sc-70164|sc-98566|sc-166809|sc-166810|sc-135868 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815). Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Sodium/hydrogen exchanger 1|
|Molecular Weight||90763 MW|
|Protein Function||Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. .|
|Tissue Specificity||Kidney and intestine.|
|Sequence Similarities||Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.|
|Subcellular Localization||Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane. .|
|Alternative Names||Sodium/hydrogen exchanger 1;APNH;Na(+)/H(+) antiporter, amiloride-sensitive;Na(+)/H(+) exchanger 1;NHE-1;Solute carrier family 9 member 1;SLC9A1;APNH1, NHE1;|
|Research Areas|||signal transduction|adapters|transmembrane| signal transduction|metabolism|plasma membrane|channels| cardiovascular|atherosclerosis|hypertension|sodium/ion transport| metabolism|types of disease|cancer|heart disease||
Background for Sodium/hydrogen exchanger 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SLC9A1/Nhe1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SLC9A1/Nhe1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti SLC9A1 (PB9151) at 0.5ug/ml
WB: Recombinant Human Sodium Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti SLC9A1 (PB9151) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
IHC(P): Rat Kidney Tissue
IHC(P): Human Mammary Cancer Tissue
IHC(P): Mouse Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,