Anti-SLIT-3 Antibody

SLIT3 antibody

Boster Bio Anti-SLIT-3 Antibody (Catalog # A04745). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Dog.

Product Info Summary

SKU: A04745
Size: 100ug
Reactive Species: Bovine, Canine, Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-SLIT-3 Antibody

View all SLIT3 Antibodies

SKU/Catalog Number

A04745

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-SLIT-3 Antibody (Catalog # A04745). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Bovine, Dog.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-SLIT-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04745)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1155-1180 of Human SLIT-3 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04745 is reactive to SLIT3 in Bovine, Canine, Human, Mouse, Rat

Applications

A04745 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

167.713kDa

Background of SLIT3

SLIT-3 (also known as multiple epidermal growth factor-like domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to SLIT3 there are two additional human 'slit' homologs, which are termed SLIT1 and SLIT2. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Slit-3 is a secreted protein predominantly expressed in thyroid. Multiple isoforms have been reported for this product.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For SLIT3 (Source: Uniprot.org, NCBI)

Gene Name

SLIT3

Full Name

Slit homolog 3 protein

Weight

167.713kDa

Alternative Names

KIAA0814; MEGF5; MEGF5Multiple epidermal growth factor-like domains protein 5; Multiple EGF-like domains protein 5; SLIL2; SLIL2FLJ10764; slit homolog 3 (Drosophila); slit homolog 3 protein; SLIT1; slit2; Slit3; Slit-3slit (Drosophila) homolog 3 SLIT3 MEGF5, SLIL2, SLIT1, Slit-3, slit2 slit guidance ligand 3 slit homolog 3 protein|multiple EGF-like domains protein 5|multiple epidermal growth factor-like domains protein 5|slit homolog 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLIT3, check out the SLIT3 Infographic

SLIT3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLIT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A04745

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-SLIT-3 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-SLIT-3 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

13 Customer Q&As for Anti-SLIT-3 Antibody

Question

I see that the anti-SLIT-3 antibody A04745 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-05-01

Question

Will A04745 anti-SLIT-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

You can see on the product datasheet, A04745 anti-SLIT-3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-14

Question

We want to test anti-SLIT-3 antibody A04745 on human testis for research purposes, then I may be interested in using anti-SLIT-3 antibody A04745 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Johnson

Verified customer

Asked: 2020-02-05

Answer

The products we sell, including anti-SLIT-3 antibody A04745, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-05

Question

Is a blocking peptide available for product anti-SLIT-3 antibody (A04745)?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

We do provide the blocking peptide for product anti-SLIT-3 antibody (A04745). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-30

Question

Is this A04745 anti-SLIT-3 antibody reactive to the isotypes of SLIT3?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

The immunogen of A04745 anti-SLIT-3 antibody is A synthetic peptide corresponding aa 1164-1177 of Human SLIT-3 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-03

Question

My question regarding product A04745, anti-SLIT-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04745 anti-SLIT-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-21

Question

See attached the WB image, lot number and protocol we used for testis using anti-SLIT-3 antibody A04745. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-03-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-08

Question

Will anti-SLIT-3 antibody A04745 work on goat WB with testis?

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

Our lab technicians have not tested anti-SLIT-3 antibody A04745 on goat. You can run a BLAST between goat and the immunogen sequence of anti-SLIT-3 antibody A04745 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-01

Question

Is there a BSA free version of anti-SLIT-3 antibody A04745 available?

Verified Customer

Verified customer

Asked: 2017-12-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SLIT-3 antibody A04745 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-21

Question

I was wanting to use your anti-SLIT-3 antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

As indicated on the product datasheet, A04745 anti-SLIT-3 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-17

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SLIT-3 antibody A04745. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-08-25

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-25

Question

Will anti-SLIT-3 antibody A04745 work for WB with testis?

R. Jha

Verified customer

Asked: 2016-08-04

Answer

According to the expression profile of testis, SLIT3 is highly expressed in testis. So, it is likely that anti-SLIT-3 antibody A04745 will work for WB with testis.

Boster Scientific Support

Answered: 2016-08-04

Question

We are currently using anti-SLIT-3 antibody A04745 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

E. Johnson

Verified customer

Asked: 2015-11-24

Answer

The anti-SLIT-3 antibody (A04745) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-24

Order DetailsPrice
A04745

100 μg

$599

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A04745
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.