Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody

SMAD1 antibody

Boster Bio Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody catalog # PA1331. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: PA1331
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody

View all SMAD1 Antibodies

SKU/Catalog Number







Boster Bio Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody catalog # PA1331. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1331)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




A synthetic peptide corresponding to a sequence at the C-terminus of human SMAD1/2/3/5 (GPLQWLDKVLTQMGS), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1331 is reactive to SMAD1 in Human, Mouse, Rat


PA1331 is guaranteed for WB Boster Guarantee

Background of SMAD1

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-beta superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For SMAD1 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Mothers against decapentaplegic homolog 1




dwarfin/SMAD family

Alternative Names

BSP1; BSP-1; hSMAD1; JV4-1SMAD, mothers against DPP homolog 1 (Drosophila); MAD homolog 1; MADH1JV41; MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila); Mad-related protein 1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMAD family member 1SMAD 1; SMAD, mothers against DPP homolog 1; Smad1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1 SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SMAD1, check out the SMAD1 Infographic

SMAD1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SMAD1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1331 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hang Liang,Qian Wang,Ding Wang,Hongwu Zheng,Dhan V. Kalvakolanu,Hua Lu,Naiyan Wen,Xuyang Chen,Libo Xu,Jiaxin Ren,Baofeng Guo,Ling Zhang, RGFP966, a histone deacetylase 3 inhibitor, promotes glioma stem cell differentiation by blocking TGF-β signaling via SMAD7,Biochemical Pharmacology,Volume 180,2020,114118,ISSN 0006-2952,https://doi. org/10.1016/j.bcp.2020.114118.
Species: Human,Mouse
PA1331 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000

Hong L,Li F,Tang C,Li L,Sun L,Li X,Zhu L.Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling.Cell Death Dis.2020 Aug 10;11(8):695.doi:10.1038/s41419-020-02818-x.PMID:32826874;PMCID:PMC7442651.
Species: Human,Mouse
PA1331 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:NA

Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, Song P, Yu Z, Liu C, Zhu Q, Zheng J. Int J Clin Exp Med. 2014 Sep 15;7(9):2645-50. Ecollection 2014. Dexamethasone Attenuates Bleomycin-Induced Lung Fibrosis In Mice Through Tgf-??, Smad3 And Jak-Stat P...

Tao Y, Hu L, Li S, Liu Q, Wu X, Li D, Fu P, Wei D, Luo Z. Transplant Proc. 2011 Jun;43(5):1985-8. Doi: 10.1016/J.Transproceed.2011.01.160. Tranilast Prevents The Progression Of Chronic Cyclosporine Nephrotoxicity Through Regulation Of Transforming...

Xu T, Ni Mm, Huang C, Meng Xm, He Yh, Zhang L, Li J. Inflammation. 2015 Oct;38(5):1794-804. Doi: 10.1007/S10753-015-0157-6. Nlrc5 Mediates Il-6 And Il-1?? Secretion In Lx-2 Cells And Modulated By The Nf-??b/Smad3 Pathway.

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4 Customer Q&As for Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody


We are currently using anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody PA1331 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-24


The anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody (PA1331) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-24


We have seen staining in human uterus. What should we do? Is anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody supposed to stain uterus positively?

Verified Customer

Verified customer

Asked: 2019-05-13


Based on literature uterus does express SMAD1. Based on, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005

Boster Scientific Support

Answered: 2019-05-13


My team were content with the WB result of your anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody. However we have observed positive staining in mammary carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-01


From literature, mammary carcinoma does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005

Boster Scientific Support

Answered: 2018-02-01


We need using your anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody for ub-specific processing proteases studies. Has this antibody been tested with western blotting on mm453 cell lysate? We would like to see some validation images before ordering.

J. Lewis

Verified customer

Asked: 2016-12-28


I appreciate your inquiry. This PA1331 anti-SMAD1/SMAD2/SMAD3/SMAD5 antibody is tested on rat heart tissue, tissue lysate, skeletal muscle tissue, kidney tissue, brain tissue, mm453 cell lysate, mm231 cell lysate, hela cell lysate, smmc cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-12-28


Total: $315


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