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SKU:PA1898
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Smad2 Antibody
SKU/Catalog Number PA1898
Description Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-Smad2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1898)
Replacement Item This antibody may replace the following items: sc-101153|sc-6200|sc-393312|sc-6200-X|sc-393312-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SMAD2
Protein Name Mothers against decapentaplegic homolog 2
Molecular Weight 52306 MW
Protein Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .
Tissue Specificity Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. .
Sequence Similarities Belongs to the dwarfin/SMAD family.
Subcellular Localization Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Uniprot ID Q15796
Alternative Names Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2;
Research Areas |signal transduction|signaling pathway|nuclear signaling|smads| epigenetics and nuclear signaling|nuclear signaling pathways| stem cells|tgf beta|cytoplasmic| cancer|growth factors|tgf|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Mothers against decapentaplegic homolog 2

Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Anti-Smad2 Antibody Images

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Anti-Smad2 Antibody
Anti-Smad2 antibody, PA1898, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD (162aa tag+D424-P578)
Lane 1: Recombinant Human SHC1 Proteins 10ng
Lane 2: Recombinant Human SHC1 Proteins 5ng
Lane 3: Recombinant Human SHC1 Proteins 2.5ng
Anti-Smad2 Antibody
Anti-Smad2 antibody, PA1898, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Smad2 Antibody
Anti-Smad2 antibody, PA1898, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: U87 Cell Lysate
Lane 6: MCF-7 Cell Lysate
Anti-Smad2 Antibody
Anti-Smad2 antibody, PA1898, ICC
ICC: HELA Cell
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Publications

Tao Y, Hu L, Li S, Liu Q, Wu X, Li D, Fu P, Wei D, Luo Z. Transplant Proc. 2011 Jun;43(5):1985-8. Doi: 10.1016/J.Transproceed.2011.01.160. Tranilast Prevents The Progression Of Chronic Cyclosporine Nephrotoxicity Through Regulation Of Transforming...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to smad2 antibody, smad antibody, mad antibody