Data & Images
|Product Name||Anti-Smad4 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 4(SMAD4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Smad4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1953)|
|Replacement Item||This antibody may replace the following items: sc-7966|sc-56479|sc-56775|sc-1908|sc-7154|sc-73599|sc-1909|sc-73040|sc-1909-R|sc-7966-X|sc-1908-X|sc-7154-X|sc-1909-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Smad4(97-113aa RLWRWPDLHKNELKHVK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mothers against decapentaplegic homolog 4|
|Molecular Weight||60439 MW|
|Protein Function||In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .|
|Sequence Similarities||Belongs to the dwarfin/SMAD family.|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta.|
|Alternative Names||Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4;|
|Research Areas|||signal transduction|signaling pathway|nuclear signaling|smads| epigenetics and nuclear signaling|nuclear signaling pathways| stem cells|tgf beta|cytoplasmic| cancer|oncoproteins/suppressors|tumor suppressors| cardiovascular|heart|apoptosis|cardiogenesis|transcription factors/regulators|hypertrophy|transcription factors|vasculature|endothelium|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes||
Background for Mothers against decapentaplegic homolog 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Smad4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Smad4 Antibody Images
Click the images to enlarge.
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue Lysate
IHC(P): Rat Brain Tissue Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,