Data & Images
|Product Name||Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)|
|Description||Mouse IgG monoclonal antibody for Smad4 (DPC4), SMAD family member 4 (SMAD4) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) DCS-46 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1089)|
|Replacement Item||This antibody may replace the following items: sc-7966|sc-56479|sc-56775|sc-1908|sc-7154|sc-73599|sc-1909|sc-73040|sc-1909-R|sc-7966-X|sc-1908-X|sc-7154-X|sc-1909-X from Santa Cruz Biotechnology.|
|Predicted Species||Bovine, Monkey|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human Smad4(DPC4).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mothers against decapentaplegic homolog 4|
|Molecular Weight||60469 MW|
|Protein Function||Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC- 3') within BMP response element (BMPRE) of cardiac activating regions. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 (By similarity). .|
|Sequence Similarities||Belongs to the dwarfin/SMAD family.|
|Subcellular Localization||Cytoplasm . Nucleus . In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation. .|
|Alternative Names||Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;SMAD family member 4;SMAD 4;Smad4;Smad4;Madh4;|
|Research Areas|||signal transduction|signaling pathway|nuclear signaling|smads| epigenetics and nuclear signaling|nuclear signaling pathways| stem cells|tgf beta|cytoplasmic| cancer|oncoproteins/suppressors|tumor suppressors| cardiovascular|heart|apoptosis|cardiogenesis|transcription factors/regulators|hypertrophy|transcription factors|vasculature|endothelium|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes||
Background for Mothers against decapentaplegic homolog 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, -|
Western blot, 2-4μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate