Data & Images
|Product Name||Anti-SNAP25 Antibody|
|Description||Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SNAP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1315)|
|Replacement Item||This antibody may replace the following items: sc-136267|sc-20038|sc-30094|sc-376713|sc-390644|sc-390874|sc-65508|sc-73044|sc-7538|sc-7539 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Synaptosomal-associated protein 25(SNAP-25)|
|Molecular Weight||23315 MW|
|Protein Function||t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. .|
|Tissue Specificity||Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.|
|Sequence Similarities||Belongs to the SNAP-25 family.|
|Subcellular Localization||Cytoplasm, perinuclear region . Cell membrane ; Lipid-anchor. Cell junction, synapse, synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). .|
|Alternative Names||Synaptosomal-associated protein 25;SNAP-25;Super protein;SUP;Synaptosomal-associated 25 kDa protein;SNAP25;SNAP;|
|Research Areas|||neuroscience|neurotransmission|secretory vesicles|snaps & snares| neuroscience| signal transduction|protein trafficking|vesicle transport|cell type marker|neuron marker|synapse marker| cancer|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|integration of energy|types of disease||
Background for Synaptosomal-associated protein 25(SNAP-25)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SNAP25 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SNAP25 Antibody Images
Click the images to enlarge.
WB: Rat Brain Tissue Lysate
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,