|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SNF5 Antibody|
|Description||Rabbit IgG polyclonal antibody for SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1(SMARCB1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SNF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1934)|
|Replacement Item||This antibody may replace the following items: sc-13055|sc-13055-X|sc-16189|sc-166164|sc-166164-X|sc-166165|sc-166165-X|sc-58381|sc-8836-R|sc-8837|sc-9749|sc-9749-X|sc-9751|sc-9751-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SNF5(362-377aa EKKIRDQDRNTRRMRR), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1|
|Molecular Weight||44141 MW|
|Protein Function||Core component of the BAF (hSWI/SNF) complex. This ATP- dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors- specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. .|
|Sequence Similarities||Belongs to the SNF5 family.|
|Alternative Names||SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1;BRG1-associated factor 47;BAF47;Integrase interactor 1 protein;SNF5 homolog;hSNF5;SMARCB1;BAF47, INI1, SNF5L1;|
|Research Areas|||epigenetics and nuclear signaling|chromatin remodeling|swi / snf| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|mediator complex||
Background for SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SNF5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SNF5 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human SMARCB1, 41.0KD (162aa tag+ L186-W385)
Lane 1: Recombinant Human SMARCB1 Protein 10ng
Lane 2: Recombinant Human SMARCB1 Protein 5ng
Lane 3: Recombinant Human SMARCB1 Protein 2.5ng
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: PC-12 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: 293T Cell Lysate
IHC(P): Rat Brain Tissue
IHC(P): Human Meningeoma Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,