|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-SOCS2 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 2(SOCS2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-SOCS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1383)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS2(181-198aa LPLPTRLKDYLEEYKFQV), identical to the related mouse and rat sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-SOCS2 antibody, PA1383, Western blotting
All lanes: Anti SOCS2 (PA1383) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HT1080 Tissue Lysate at 50ug
Lane 7: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 8: Mouse Liver Tissue Lysate at 50ug
Lane 9: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Suppressor of cytokine signaling 2|
|Tissue Specificity||High expression in heart, placenta, lung, kidney and prostate.|
|Alternative Names||Suppressor of cytokine signaling 2;SOCS-2;Cytokine-inducible SH2 protein 2;CIS-2;STAT-induced STAT inhibitor 2;SSI-2;SOCS2;CIS2, SSI2, STATI2;|
|Molecular Weight||22172 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Suppressor of cytokine signaling 2 is a protein that in humans is encoded by the SOCS2 gene. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signalling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon-gamma(IFN-gamma). The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor 1 receptor(IGF1R), and thus is though to be involved in the regulation of IGF1R mediated cell signaling. Knockout studies in mice also suggested a regulatory role of this gene in IGF-1 related growth control. By cytogenetic and radiation hybrid mapping, Yandava et al.(1999) mapped the SOCS2 gene to chromosome 12q21.3-q23.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at email@example.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.