|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SOCS2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 2(SOCS2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-SOCS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1383)|
|Replacement Item||This antibody may replace the following items: sc-7007|sc-7007-R|sc-7008|sc-9022 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SOCS2(181-198aa LPLPTRLKDYLEEYKFQV), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Suppressor of cytokine signaling 2(SOCS-2)|
|Molecular Weight||22172 MW|
|Protein Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.|
|Tissue Specificity||High expression in heart, placenta, lung, kidney and prostate.|
|Sequence Similarities||Contains 1 SH2 domain.|
|Alternative Names||Suppressor of cytokine signaling 2;SOCS-2;Cytokine-inducible SH2 protein 2;CIS-2;STAT-induced STAT inhibitor 2;SSI-2;SOCS2;CIS2, SSI2, STATI2;|
|Research Areas|||immunology|innate immunity|cytokines|il6 related| neuroscience|neurology process|neural signal transduction| signal transduction|adapters|cytoplasmic||
Background for Suppressor of cytokine signaling 2(SOCS-2)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SOCS2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SOCS2 Antibody Images
Click the images to enlarge.
All lanes: Anti SOCS2 (PA1383) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: HT1080 Tissue Lysate at 50ug
Lane 7: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 8: Mouse Liver Tissue Lysate at 50ug
Lane 9: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,