Anti-SOD (EC) Monoclonal Antibody

SOD3/EC-SOD antibody

Boster Bio Anti-SOD (EC) Monoclonal Antibody catalog # M01784. Tested in ELISA, IF, ICC applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01784
Size: 100ug
Reactive Species: Guinea pig, Human, Mouse, Rat
Host: Mouse
Application: ELISA, IF, IHC, ICC, WB

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Product Name

Anti-SOD (EC) Monoclonal Antibody

View all SOD3/EC-SOD Antibodies

SKU/Catalog Number

M01784

Size

100ug

Form

liquid

Description

Boster Bio Anti-SOD (EC) Monoclonal Antibody catalog # M01784. Tested in ELISA, IF, ICC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SOD (EC) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01784)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

4GG11G6

Isotype

IgG1 Kappa

Immunogen

Human extracellular SOD purified from aortas

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects extracellular SOD ~35kDa.

Reactive Species

M01784 is reactive to SOD3 in Guinea pig, Human, Mouse, Rat

Applications

M01784 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

25.851kDa

Background of SOD3/EC-SOD

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For SOD3 (Source: Uniprot.org, NCBI)

Gene Name

SOD3

Full Name

Extracellular superoxide dismutase [Cu-Zn]

Weight

25.851kDa

Superfamily

Cu-Zn superoxide dismutase family

Alternative Names

ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular SOD3 EC-SOD superoxide dismutase 3 extracellular superoxide dismutase [Cu-Zn]|superoxide dismutase 3, extracellular|testicular tissue protein Li 175

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SOD3, check out the SOD3 Infographic

SOD3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SOD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01784 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Epigallocatechin%u20103%u2010Gallate Attenuates Oxidative Stress and Inflammation in Obstructive Nephropathy via NF%u2010%u03BAB and Nrf2/HO%u20101 Signalling Pathway Regulation

Long Sh, Yu Zq, Shuai L, Guo Yl, Duan Dl, Xu Xy, Li Xd. Int J Mol Sci. 2012;13(3):3354-65. Doi: 10.3390/Ijms13033354. Epub 2012 Mar 12. The Hypoglycemic Effect Of The Kelp On Diabetes Mellitus Model Induced By Alloxan In Rats.

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Order DetailsPrice
M01784

100 μg

$407

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