Data & Images
|Product Name||Anti-SP100 Antibody|
|Description||Rabbit IgG polyclonal antibody for Nuclear autoantigen Sp-100(SP100) detection. Tested with WB in Human.|
|Cite This Product||Anti-SP100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2286)|
|Replacement Item||This antibody may replace the following items: sc-16328|sc-25568 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SP100(664-677aa YTLKVLMENKFLPE).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Nuclear autoantigen Sp-100|
|Molecular Weight||100417 MW|
|Protein Function||Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2 according to PubMed:11909962. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA according to PubMed:15247905. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53- mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. Also plays a role in infection by viruses, including human cytomegalovirus and Epstein-Barr virus, through mechanisms that may involve chromatin and/or transcriptional regulation. .|
|Tissue Specificity||Widely expressed. Sp100-B is expressed only in spleen, tonsil, thymus, mature B-cell line and some T-cell line, but not in brain, liver, muscle or non-lymphoid cell lines.|
|Sequence Similarities||Contains 2 HMG box DNA-binding domains.|
|Subcellular Localization||Nucleus. Nucleus, PML body. Cytoplasm. Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon- dependent. Accumulates in the cytoplasm upon FAS activation.|
|Alternative Names||Nuclear autoantigen Sp-100;Nuclear dot-associated Sp100 protein;Speckled 100 kDa;SP100;|
|Research Areas|||immunology|immune system diseases|autoimmune| tags & cell markers|subcellular markers|nucleus|other nuclear bodies| epigenetics and nuclear signaling|chromatin binding proteins|dna / rna binding||
Background for Nuclear autoantigen Sp-100
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SP100 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SP100 Antibody Images
Click the images to enlarge.
All lanes: Anti-SP100(PA2286) at 0.5ug/ml
Lane 1: U937 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 100KD
Observed bind size: 100KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,