Product Info Summary
SKU: | A08730-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SP6 Antibody Picoband™
SKU/Catalog Number
A08730-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SP6 Antibody Picoband™ catalog # A08730-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SP6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08730-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SP6, which shares 97.4% amino acid (aa) sequence identity with both mouse and rat SP6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08730-1 is reactive to SP6 in Human, Mouse, Rat
Applications
A08730-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
39.84kDa
Background of SP6
SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes. By somatic cell hybrid analysis and FISH, he SP6 gene is mapped t to chromosome 17q21.3-q22.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SP6 using anti-SP6 antibody (A08730-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human MCF-7 cell lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 45KD. The expected band size for SP6 is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For SP6 (Source: Uniprot.org, NCBI)
Gene Name
SP6
Full Name
Transcription factor Sp6
Weight
39.84kDa
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
Epfn; EPIPROFIN; KLF14MGC119663; Krueppel-like factor 14; Kruppel-like factor 14; MGC119662; MGC119664; MGC119665; Sp6 transcription factor; transcription factor Sp6 SP6 EPFN, EPIPROFIN, KLF14 Sp6 transcription factor transcription factor Sp6|Kruppel-like factor 14|krueppel-like factor 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SP6, check out the SP6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SP6 Antibody Picoband™ (A08730-1)
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No publications found for A08730-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-SP6 Antibody Picoband™
Question
We are currently using anti-SP6 antibody A08730-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
The anti-SP6 antibody (A08730-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15