Anti-SPHK1 Monoclonal Antibody

Sphingosine Kinase 1/SPHK1 antibody

Boster Bio Anti-SPHK1 Monoclonal Antibody catalog # M01390. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01390
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-SPHK1 Monoclonal Antibody

View all Sphingosine Kinase 1/SPHK1 Antibodies

SKU/Catalog Number

M01390

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-SPHK1 Monoclonal Antibody catalog # M01390. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SPHK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01390)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACCB-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human SPHK1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01390 is reactive to SPHK1 in Human, Mouse, Rat

Applications

M01390 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

120-140 kDa

Calculated molecular weight

42.518kDa

Background of Sphingosine Kinase 1/SPHK1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For SPHK1 (Source: Uniprot.org, NCBI)

Gene Name

SPHK1

Full Name

Sphingosine kinase 1

Weight

42.518kDa

Alternative Names

EC 2.7.1.91; SK1; sphingosine kinase 1; SPHK1; SPHKSK 1; SPK 1; SPK; SPK1 SPHK1 SPHK sphingosine kinase 1 sphingosine kinase 1|SK 1|SPK 1|acetyltransferase SPHK1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SPHK1, check out the SPHK1 Infographic

SPHK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPHK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01390 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen C, Gong W, Li C, Xiong F, Wang S, Huang J, Wang Y, Chen Z, Chen Q, Liu P, Lan T, Huang H. Oncotarget. 2017 Aug 10;8(45):78660-78676. doi: 10.18632/oncotarget.20205. eCollection 2017 Oct 3. Sphingosine kinase 1 mediates AGEs-induced fibronecti...

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1 Customer Q&As for Anti-SPHK1 Monoclonal Antibody

Question

What is the sample target on Western blot analysis in your product image for M01390?

Verified customer

Asked: 2019-12-09

Answer

Western blot analysis of the Anti-SPHK1 Monoclonal Antibody (M01390) expression in Raji cell lysate.

Boster Scientific Support

Answered: 2019-12-10

Order DetailsPrice
M01390

100μL

$370
M01390-30ul

30μL sample

$99
M01390-carrier-free

Carrier Free

$370

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M01390
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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