|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-SPHK1/Sphingosine Kinase 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Sphingosine kinase 1(SPHK1) detection. Tested with WB, IHC-P, ICC in Human;Mouse.|
|Cite This Product||Anti-SPHK1/Sphingosine Kinase 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1996)|
|Replacement Item||This antibody may replace the following items: sc-22702|sc-365401|sc-48825|sc-34528|sc-100441 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SPHK1(117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Sphingosine kinase 1|
|Molecular Weight||42518 MW|
|Protein Function||Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. .|
|Tissue Specificity||Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. .|
|Subcellular Localization||Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.|
|Alternative Names||Sphingosine kinase 1;SK 1;SPK 1;22.214.171.124;SPHK1;SPHK, SPK;|
|Research Areas|||signal transduction|signaling pathway|lipid signaling|lipid kinases| signal transduction|g protein signaling|gpcr| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|types of disease||
Background for Sphingosine kinase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SPHK1/Sphingosine Kinase 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SPHK1/Sphingosine Kinase 1 Antibody Images
Click the images to enlarge.
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: Hut Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
IHC(P): Human Placenta Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,