Product Info Summary
SKU: | A01382 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Sphingosine kinase 2 SphK2 Antibody
View all Sphingosine Kinase 2/SPHK2 Antibodies
SKU/Catalog Number
A01382
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Sphingosine kinase 2 SphK2 Antibody catalog # A01382. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sphingosine kinase 2 SphK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01382)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SphK2 around the non-phosphorylation site of T614.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01382 is reactive to Sphk2 in Human, Mouse, Rat
Applications
A01382 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
65.618kDa
Background of Sphingosine Kinase 2/SPHK2
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of AD293 using SphK2 Polyclonal antibody.
Click image to see more details
Western Blot (WB) analysis of 22RV1 cells using SphK2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For Sphk2 (Source: Uniprot.org, NCBI)
Gene Name
Sphk2
Full Name
Sphingosine kinase 2
Weight
65.618kDa
Alternative Names
EC 2.7.1.91; SK 2; SK2; sphingosine kinase 2; sphingosine kinase type 2 isoform; SPHK2; SPK 2; SPK2 Sphk2|C76851, Sk2, Spk2|sphingosine kinase 2|sphingosine kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sphk2, check out the Sphk2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sphk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sphingosine kinase 2 SphK2 Antibody (A01382)
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No publications found for A01382
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