Click image to see more details
Product Info Summary
| SKU: | A03254-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SPINK5 Antibody Picoband®
SKU/Catalog Number
A03254-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPINK5 Antibody Picoband® catalog # A03254-2. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SPINK5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03254-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse SPINK5 recombinant protein (Position: H191-N506). Mouse SPINK5 shares 59.8% amino acid (aa) sequence identity with human SPINK5.
Reactive Species
A03254-2 is reactive to SPINK5 in Mouse, Rat
Observed Molecular Weight
120 kDa
Background of SPINK5
Enables peptidase inhibitor activity. Acts upstream of or within negative regulation of serine-type peptidase activity and regulation of cell adhesion. Predicted to be located in several cellular components, including endoplasmic reticulum membrane; epidermal lamellar body; and perinuclear region of cytoplasm. Predicted to be active in extracellular region. Is expressed in back skin. Used to study Netherton syndrome. Human ortholog(s) of this gene implicated in Netherton syndrome. Orthologous to human SPINK5 (serine peptidase inhibitor Kazal type 5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03254-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SPINK5 using anti-SPINK5 antibody (A03254-2).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse stomach tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK5 antigen affinity purified polyclonal antibody (A03254-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPINK5 at approximately 120 kDa. The expected band size for SPINK5 is at 121 kDa.
Specific Publications For Anti-SPINK5 Antibody Picoband® (A03254-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SPINK5 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SPINK5 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
