Data & Images
|Product Name||Anti-SPTLC1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Serine palmitoyltransferase 1(SPTLC1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-SPTLC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1777)|
|Replacement Item||This antibody may replace the following items: sc-27495|sc-32916|sc-374143|sc-27497|sc-136076 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1(138-154aa FYGTFDVHLDLEDRLAK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Serine palmitoyltransferase 1|
|Molecular Weight||52744 MW|
|Protein Function||Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. .|
|Tissue Specificity||Widely expressed. Not detected in small intestine. .|
|Sequence Similarities||Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.|
|Subcellular Localization||Endoplasmic reticulum membrane ; Single-pass membrane protein .|
|Alternative Names||Serine palmitoyltransferase 1;22.214.171.124;Long chain base biosynthesis protein 1;LCB 1;Serine-palmitoyl-CoA transferase 1;SPT 1;SPT1;SPTLC1;LCB1;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| neuroscience|neurology process|neurodegenerative disease| signal transduction| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism||
Background for Serine palmitoyltransferase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SPTLC1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SPTLC1 Antibody Images
Click the images to enlarge.
All lanes: Anti SPTLC1 (PA1777) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: S549 Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,