Anti-Src Monoclonal Antibody (5A18)

SRC antibody

Mouse Monoclonal 5A18 antibody for SRC detection. Tested positive for IHC, WB in Human, Mouse, Rat Cited in 1 publication(s).

Product Info Summary

SKU: M00107-2
Size: 100µg
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

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Product Name

Anti-Src Monoclonal Antibody (5A18)

View all SRC Antibodies

SKU/Catalog Number

M00107-2

Size

100µg

Form

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Description

Mouse Monoclonal 5A18 antibody for SRC detection. Tested positive for IHC, WB in Human, Mouse, Rat

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Src Monoclonal Antibody (5A18) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00107-2)

Host

Mouse

Contents

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

5A18

Isotype

IgG2b, kappa

Immunogen

Recombinant human full length c-Src.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00107-2 is reactive to SRC in Human, Mouse, Rat

Applications

M00107-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

59.835kDa

Background of SRC

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

Gene/Protein Information For SRC (Source: Uniprot.org, NCBI)

Gene Name

SRC

Full Name

Proto-oncogene tyrosine-protein kinase Src

Weight

59.835kDa

Superfamily

protein kinase superfamily

Alternative Names

ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) SRC ASV1, THC6, c-SRC, p60-Src, SRC SRC proto-oncogene, non-receptor tyrosine kinase proto-oncogene tyrosine-protein kinase Src|proto-oncogene c-Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1|v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SRC, check out the SRC Infographic

SRC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00107-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W...

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15 Customer Q&As for Anti-Src Monoclonal Antibody (5A18)

Question

We have observed staining in mouse platelet. Do you have any suggestions? Is anti-Src Monoclonal antibody (5A18) supposed to stain platelet positively?

K. Mitchell

Verified customer

Asked: 2020-02-21

Answer

From what I have seen in literature platelet does express SRC. From what I have seen in Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2020-02-21

Question

I see that the anti-Src Monoclonal antibody (5A18) M00107-2 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-11

Question

Please see the WB image, lot number and protocol we used for fundus of stomach using anti-Src Monoclonal antibody (5A18) M00107-2. Please let me know if you require anything else.

R. Dhar

Verified customer

Asked: 2019-09-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-18

Question

I am looking for to test anti-Src Monoclonal antibody (5A18) M00107-2 on rat fundus of stomach for research purposes, then I may be interested in using anti-Src Monoclonal antibody (5A18) M00107-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

The products we sell, including anti-Src Monoclonal antibody (5A18) M00107-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-18

Question

We purchased anti-Src Monoclonal antibody (5A18) for WB on leukemic t-cell in the past. I am using mouse, and We intend to use the antibody for IHC next. I would like examining leukemic t-cell as well as lung skin in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

I looked at the website and datasheets of our anti-Src Monoclonal antibody (5A18) and it seems that M00107-2 has been tested on mouse in both WB and IHC. Thus M00107-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-05-16

Question

Is there a BSA free version of anti-Src Monoclonal antibody (5A18) M00107-2 available?

N. Jackson

Verified customer

Asked: 2018-07-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Src Monoclonal antibody (5A18) M00107-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-26

Question

We are currently using anti-Src Monoclonal antibody (5A18) M00107-2 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

The anti-Src Monoclonal antibody (5A18) (M00107-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-12

Question

Is a blocking peptide available for product anti-Src Monoclonal antibody (5A18) (M00107-2)?

T. Mitchell

Verified customer

Asked: 2017-02-23

Answer

We do provide the blocking peptide for product anti-Src Monoclonal antibody (5A18) (M00107-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-23

Question

I was wanting to use your anti-Src Monoclonal antibody (5A18) for IHC for rat fundus of stomach on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fundus of stomach identification?

H. Taylor

Verified customer

Asked: 2017-02-09

Answer

As indicated on the product datasheet, M00107-2 anti-Src Monoclonal antibody (5A18) has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fundus of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-02-09

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fundus of stomach using anti-Src Monoclonal antibody (5A18) M00107-2. Let me know if you need anything else.

D. Mangal

Verified customer

Asked: 2017-01-09

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-01-09

Question

Does anti-Src Monoclonal antibody (5A18) M00107-2 work for IHC with fundus of stomach?

N. Edwards

Verified customer

Asked: 2016-10-10

Answer

According to the expression profile of fundus of stomach, SRC is highly expressed in fundus of stomach. So, it is likely that anti-Src Monoclonal antibody (5A18) M00107-2 will work for IHC with fundus of stomach.

Boster Scientific Support

Answered: 2016-10-10

Question

Our lab were happy with the WB result of your anti-Src Monoclonal antibody (5A18) . However we have observed positive staining in erythroleukemia cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?

M. Carter

Verified customer

Asked: 2016-04-01

Answer

From literature, erythroleukemia does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2016-04-01

Question

Is this M00107-2 anti-Src Monoclonal antibody (5A18) reactive to the isotypes of SRC?

N. Zhang

Verified customer

Asked: 2014-04-17

Answer

The immunogen of M00107-2 anti-Src Monoclonal antibody (5A18) is Recombinant human full length c-Src. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-04-17

Question

Would M00107-2 anti-Src Monoclonal antibody (5A18) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Taylor

Verified customer

Asked: 2013-08-02

Answer

You can see on the product datasheet, M00107-2 anti-Src Monoclonal antibody (5A18) as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-08-02

Question

I have a question about product M00107-2, anti-Src Monoclonal antibody (5A18) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Parker

Verified customer

Asked: 2013-03-25

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00107-2 anti-Src Monoclonal antibody (5A18) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-03-25

Order DetailsPrice
M00107-2-100ug

100 μg

$487

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