Product Info Summary
SKU: | M00107 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Src Rabbit Monoclonal Antibody
SKU/Catalog Number
M00107
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Src Rabbit Monoclonal Antibody catalog # M00107. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Src Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00107)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
III-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Src
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00107 is reactive to SRC in Human, Mouse, Rat
Applications
M00107 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
59.835kDa
Background of SRC
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Src expression in Hela cell lysate.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SRC (Source: Uniprot.org, NCBI)
Gene Name
SRC
Full Name
Proto-oncogene tyrosine-protein kinase Src
Weight
59.835kDa
Superfamily
protein kinase superfamily
Alternative Names
ASV; c-Src; EC 2.7.10; EC 2.7.10.2; pp60c-src; Rous sarcoma; RSVgp4; Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) SRC ASV1, THC6, c-SRC, p60-Src, SRC SRC proto-oncogene, non-receptor tyrosine kinase proto-oncogene tyrosine-protein kinase Src|proto-oncogene c-Src|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1|v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRC, check out the SRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Src Rabbit Monoclonal Antibody (M00107)
Hello CJ!
M00107 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Qq, Zhou Dl, Lei Y, Zheng L, Chen Sx, Gou Hj, Gu Ql, He Xd, Lan T, Qi Cl, Li Jc, Ding Yq, Qiao L, Wang Lj. Oncotarget. 2015 Feb 20;6(5):3123-35. Slit2/Robo1 Signaling Promotes Intestinal Tumorigenesis Through Src-Mediated Activation Of The W...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Src Rabbit Monoclonal Antibody
Question
We are currently using anti-Src Rabbit Monoclonal antibody M00107 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
The anti-Src Rabbit Monoclonal antibody (M00107) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-12
Question
We have been able to see staining in human fundus of stomach. What should we do? Is anti-Src Rabbit Monoclonal antibody supposed to stain fundus of stomach positively?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Based on literature fundus of stomach does express SRC. Based on Uniprot.org, SRC is expressed in fundus of stomach, lung skin, platelet, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-11-13
Question
We are interested in using your anti-Src Rabbit Monoclonal antibody for negative regulation of cysteine-type endopeptidase activity involved in apoptotic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
Thank you for your inquiry. This M00107 anti-Src Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-06-15
Question
Our lab were satisfied with the WB result of your anti-Src Rabbit Monoclonal antibody. However we have seen positive staining in leukemic t-cell cell membrane. using this antibody. Is that expected? Could you tell me where is SRC supposed to be expressed?
S. Li
Verified customer
Asked: 2014-11-28
Answer
From literature, leukemic t-cell does express SRC. Generally SRC expresses in cell membrane. Regarding which tissues have SRC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2014-11-28
Question
We bought anti-Src Rabbit Monoclonal antibody for WB on fundus of stomach in the past. I am using mouse, and We want to use the antibody for IF next. I am interested in examining fundus of stomach as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Jones
Verified customer
Asked: 2014-07-03
Answer
I took a look at the website and datasheets of our anti-Src Rabbit Monoclonal antibody and it appears that M00107 has been tested on mouse in both WB and IF. Thus M00107 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-03