|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SRC3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Nuclear receptor coactivator 3(NCOA3) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-SRC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2277)|
|Replacement Item||This antibody may replace the following items: sc-13066|sc-13066-X|sc-14605|sc-14605-X|sc-14607|sc-14607-X|sc-166751|sc-166751-X|sc-25742|sc-25742-X|sc-32348|sc-32348-X|sc-33671|sc-5305|sc-5305-X|sc-56854|sc-56855|sc-56856|sc-71654|sc-71657|sc-7216|sc-7216-X|sc-7217|sc-7217-X|sc-80992|sc-9119|sc-9119-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SRC3(1043-1061aa NLEGQSDERALLDQLHTLL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid..|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Nuclear receptor coactivator 3|
|Molecular Weight||155293 MW|
|Protein Function||Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.|
|Tissue Specificity||Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.|
|Sequence Similarities||Belongs to the SRC/p160 nuclear receptor coactivator family.|
|Subcellular Localization||Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.|
|Alternative Names||Nuclear receptor coactivator 3;NCoA-3;220.127.116.11;ACTR;Amplified in breast cancer 1 protein;AIB-1;CBP-interacting protein;pCIP;Class E basic helix-loop-helix protein 42;bHLHe42;Receptor-associated coactivator 3;RAC-3;Steroid receptor coactivator protein 3;SRC-3;Thyroid hormone receptor activator molecule 1;TRAM-1;NCOA3;AIB1, BHLHE42, RAC3, TRAM1;|
|Research Areas|||epigenetics and nuclear signaling|chromatin modifying enzymes|acetylation| signal transduction|signaling pathway|nuclear hormone receptors|co-activators/co-repressors| epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors|hat| cancer|coactivators and repressors|chip'ing antibodies|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|types of disease|obesity||
Background for Nuclear receptor coactivator 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SRC3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SRC3 Antibody Images
Click the images to enlarge.
All lanes: Anti-NCOA3(PA2277) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,