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SKU:PA2277
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-SRC3 Antibody
SKU/Catalog Number PA2277
Description Rabbit IgG polyclonal antibody for Nuclear receptor coactivator 3(NCOA3) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-SRC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2277)
Replacement Item This antibody may replace the following items: sc-13066|sc-13066-X|sc-14605|sc-14605-X|sc-14607|sc-14607-X|sc-166751|sc-166751-X|sc-25742|sc-25742-X|sc-32348|sc-32348-X|sc-33671|sc-5305|sc-5305-X|sc-56854|sc-56855|sc-56856|sc-71654|sc-71657|sc-7216|sc-7216-X|sc-7217|sc-7217-X|sc-80992|sc-9119|sc-9119-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human SRC3(1043-1061aa NLEGQSDERALLDQLHTLL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid..
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NCOA3
Protein Name Nuclear receptor coactivator 3
Molecular Weight 155293 MW
Protein Function Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Tissue Specificity Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Sequence Similarities Belongs to the SRC/p160 nuclear receptor coactivator family.
Subcellular Localization Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Uniprot ID Q9Y6Q9
Alternative Names Nuclear receptor coactivator 3;NCoA-3;2.3.1.48;ACTR;Amplified in breast cancer 1 protein;AIB-1;CBP-interacting protein;pCIP;Class E basic helix-loop-helix protein 42;bHLHe42;Receptor-associated coactivator 3;RAC-3;Steroid receptor coactivator protein 3;SRC-3;Thyroid hormone receptor activator molecule 1;TRAM-1;NCOA3;AIB1, BHLHE42, RAC3, TRAM1;
Research Areas |epigenetics and nuclear signaling|chromatin modifying enzymes|acetylation| signal transduction|signaling pathway|nuclear hormone receptors|co-activators/co-repressors| epigenetics and nuclear signaling|nuclear signaling pathways|nuclear receptors|hat| cancer|coactivators and repressors|chip'ing antibodies|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|types of disease|obesity|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nuclear receptor coactivator 3

The nuclear receptor coactivator 3 also known as NCOA3 is a protein that, in humans, is encoded by the NCOA3 gene. It is mapped to 20q13.12. This gene was amplified and overexpressed in breast and ovarian cancer cell lines, as well as in breast cancer biopsies, and altered NCOA3 expression may contribute to development of steroid-dependent cancers. NCOA3 is a transcriptional coactivator protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. In addition to those, this gene is recruited to DNA promotion sites by ligand-activated nuclear receptors. NCOA3, in turn, acylates histones, which makes downsteam DNA more accessible to transcription. Hence, NCOA3 assists nuclear receptors in the upregulation of gene expression.

Anti-SRC3 Antibody Images

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Anti-SRC3 Antibody
Anti-SRC3 antibody, PA2277, All Western blotting
All lanes: Anti-NCOA3(PA2277) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to ncoa3 antibody, rac3 antibody, src3 antibody, tram1 antibody