|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Stanniocalcin 1/STC1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Stanniocalcin-1(STC1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Stanniocalcin 1/STC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1997)|
|Replacement Item||This antibody may replace the following items: sc-30183|sc-23270|sc-14346 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Stanniocalcin 1(150-167aa QLPNHFSNRYYNRLVRSL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||27621 MW|
|Protein Function||Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.|
|Tissue Specificity||Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.|
|Research Areas|||signal transduction|growth factors/hormones| signal transduction|metabolism|vitamins / minerals| cell biology|other antibodies|oxidative stress| metabolism|pathways and processes|cofactors, vitamins / minerals|redox metabolism|types of disease|cancer||
Background for Stanniocalcin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Stanniocalcin 1/STC1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Stanniocalcin 1/STC1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: Rat Stomach Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: SW620 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,