|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-STAT1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 1-alpha/beta(STAT1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-STAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1075)|
|Replacement Item||This antibody may replace the following items: sc-135648|sc-136229|sc-16570|sc-16570-R|sc-16570-X|sc-271661|sc-345|sc-345-X|sc-346|sc-346-G|sc-346-X|sc-398524|sc-398524-X|sc-417|sc-417-X|sc-464|sc-464-X|sc-51700|sc-51702|sc-53607|sc-591|sc-591-X|sc-592|sc-592-X|sc-71791|sc-73070|sc-7988|sc-7988-R|sc-7988-X|sc-81522|sc-8394|sc-8394-X|sc-98783 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human STAT1(364-378aa FDKDVNERNTVKGFR), different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Signal transducer and activator of transcription 1-alpha/beta|
|Molecular Weight||87335 MW|
|Protein Function||Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. .|
|Sequence Similarities||Belongs to the transcription factor STAT family.|
|Subcellular Localization||Cytoplasm . Nucleus . Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4.|
|Alternative Names||Signal transducer and activator of transcription 1-alpha/beta;Transcription factor ISGF-3 components p91/p84;STAT1;|
|Research Areas|||signal transduction|signaling pathway|nuclear signaling|stats| epigenetics and nuclear signaling|transcription|other factors|nuclear signaling pathways| cancer|stat family||
Background for Signal transducer and activator of transcription 1-alpha/beta
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-STAT1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-STAT1 Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 1: Mouse Heart Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Kidney Tissue Lysate
Lane 5: Mouse Spleen Tissue Lysate
Lane 6: Mouse Thymus Tissue Lysate
Lane 7: Mouse Lung Tissue Lysate
Lane 8: Mouse Intestine Tissue Lysate
Lane 9: Mouse Ovary Tissue Lysate
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- STAT1 antigen affinity purified polyclonal antibody (Catalog # PA1075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,