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Product Info Summary
| SKU: | M00312-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-STIM1 Purified Monoclonal Antibody
SKU/Catalog Number
M00312-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-STIM1 Purified Monoclonal Antibody (Catalog# M00312-1). Tested in IP, WB, IHC-P, ICC application(s). This antibody reacts with Rat, Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-STIM1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00312-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CDN3H4
Isotype
Mouse IgG1
Immunogen
Synthetized peptide (C-terminal cytoplasmic part of STIM1). The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Reactive Species
M00312-1 is reactive to STIM1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Background of STIM1
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00312-1 is guaranteed for IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): 5 μg/ml.
Western blotting: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
Validation Images & Assay Conditions
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Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line.
Lane 1: immunostaining with anti-STIM1 (CDN3H4)
Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)
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Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).
Specific Publications For Anti-STIM1 Purified Monoclonal Antibody (M00312-1)
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